BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5841

Title: 15N T1 and T2 relaxation rates, 1H{15N} NOE, and Hydrogen/Deuterium exchange data of kinase-interacting FHA domain of Arabidopsis kinase associasted protein phosphatase   PubMed: 12652139

Authors: Ding, Zhaofeng; Lee, Gui-in; Liang, Xiangyang; Gallazzi, Fabio; Van Doren, Steven

Citation: Lee, Gui-in; Li, Jia; Walker, John; Van Doren, Steven. "1H, (13)C and (15)N Resonance Assignments of the Kinase-interacting FHA Domain of Arabidopsis thaliana Kinase-associated Protein Phophatase"  J. Biomol. NMR 25, 253-254 (2003).

Assembly members:
kinase associated protein phosphatase, polymer, 139 residues, Formula weight is not available

Natural source:   Common Name: Thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
kinase associated protein phosphatase: GPLGSSWLFLEVIAGPAIGL QHAVNSTSSSKLPVKLGRVS PSDLALKDSEVSGKHAQITW NSTKFKWELVDMGSLNGTLV NSHSISHPDLGSRKWGNPVE LASDDIITLGTTTKVYVRIS SQNEFQIPFKIGVASDPMA

Data sets:
Data typeCount
T1 relaxation values215
T2 relaxation values208
order parameters104
H exchange rates43

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Kinase-interacting FHA domain of KAPP1

Entities:

Entity 1, Kinase-interacting FHA domain of KAPP 139 residues - Formula weight is not available

1   GLYPROLEUGLYSERSERTRPLEUPHELEU
2   GLUVALILEALAGLYPROALAILEGLYLEU
3   GLNHISALAVALASNSERTHRSERSERSER
4   LYSLEUPROVALLYSLEUGLYARGVALSER
5   PROSERASPLEUALALEULYSASPSERGLU
6   VALSERGLYLYSHISALAGLNILETHRTRP
7   ASNSERTHRLYSPHELYSTRPGLULEUVAL
8   ASPMETGLYSERLEUASNGLYTHRLEUVAL
9   ASNSERHISSERILESERHISPROASPLEU
10   GLYSERARGLYSTRPGLYASNPROVALGLU
11   LEUALASERASPASPILEILETHRLEUGLY
12   THRTHRTHRLYSVALTYRVALARGILESER
13   SERGLNASNGLUPHEGLNILEPROPHELYS
14   ILEGLYVALALASERASPPROMETALA

Samples:

sample_one: kinase associated protein phosphatase, [U-99% 15N], 0.6 mM; NaCl 120 mM

condition_one: pH: 6.3; temperature: 298 K; ionic strength: 0.12 M

condition_two: pH: 6.3; temperature: 293 K; ionic strength: 0.12 M

Experiments:

NameSampleSample stateSample conditions
15N T1 relaxationsample_onenot availablenot available
15N T2 relaxationsample_onenot availablenot available
heteronuclear 1H-15N NOEsample_onenot availablenot available
1H-15N HSQCsample_onenot availablenot available

Software:

Sybyl TRIAD v6.3 - data processing

Kaleidograph for windows v3.08 - R1, R2, Hydrogen exchange data fitting

Modelfree v4.1 - for optimizing 'Lipari-Szabo model free' parameters to heteronuclear relaxation data

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Varian INOVA 600 MHz

Related Database Links:

REF NP_197429
SWISS-PROT P46014
GenBank AAK76527 AAM51227 AAB38148
PDB
BMRB 5564 6474