BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5847

Title: Backbone and sidechain 1H, 13C, and 15N chemical shifts for Sda   PubMed: 15023339

Authors: Rowland, Susan; Maciejewski, Mark; King, Glenn

Citation: Rowland, Susan; Burkholder, William; Cunningham, K; Maciejewski, Mark; Grossman, Alan; King, Glenn. "Structure and mechanism of action of Sda, an inhibitor of the histidine kinases that regulate initiation of sporulation in Bacillus subtilis"  Mol. Cell. 13, 689-701 (2004).

Assembly members:
Sda, polymer, 46 residues, Formula weight is not available

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Sda: MRKLSDELLIESYFKATEMN LNRDFIELIENEIKRRSLGH IISVSS

Data sets:
Data typeCount
1H chemical shifts353
13C chemical shifts201
15N chemical shifts49

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Sda1

Entities:

Entity 1, Sda 46 residues - Formula weight is not available

1   METARGLYSLEUSERASPGLULEULEUILE
2   GLUSERTYRPHELYSALATHRGLUMETASN
3   LEUASNARGASPPHEILEGLULEUILEGLU
4   ASNGLUILELYSARGARGSERLEUGLYHIS
5   ILEILESERVALSERSER

Samples:

Sample_1: Sda, [U-13C; U-15N], 1.0 mM

cond_H2O: pH: 6.85 na; temperature: 298 K; ionic strength: 0.125 M

cond_D2O: pH: 6.85 na; temperature: 298 K; ionic strength: 0.125 mM

Experiments:

NameSampleSample stateSample conditions
3D HNCACBnot availablenot availablenot available
3D CBCA(CO)NHnot availablenot availablenot available
3D HC(CO)NH-TOCSYnot availablenot availablenot available
3D C(CO)NH-TOCSYnot availablenot availablenot available
3D HNCOnot availablenot availablenot available
3D HNHAnot availablenot availablenot available
3D HNHBnot availablenot availablenot available
3D HCCH-COSYnot availablenot availablenot available
2D 1H-1H TOCSYnot availablenot availablenot available
2D 1H-1H DQF-COSYnot availablenot availablenot available
3D 15N-separated NOESY-HSQCnot availablenot availablenot available
3D 13C-separated NOESY-HSQCnot availablenot availablenot available
3D 15N-separated TOCSY-HSQCnot availablenot availablenot available

Software:

NMRpipe v1.0 - NMR data processing

XEASY v1.3.13 - Spectral analysis, peak assignment

NMR spectrometers:

  • INOVA Varian 500 MHz
  • INOVA Varian 600 MHz

Related Database Links:

PDB
DBJ BAI86094 GAK78421
EMBL CAE01463 CCU59089
GB ADM38587 ADP33164 ADV93314 AEP87448 AEP91604
REF WP_003226124 WP_003325380 WP_010886571 YP_003866896 YP_003974095
SP Q7WY62