BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5848

Title: Solution structure of S8-SP11   PubMed: 12835321

Authors: Mishima, M.; Takayama, S.; Sasaki, K.; Jee, J.; Kojima, C.; Isogai, A.; Shirakawa, M.

Citation: Mishima, M.; Takayama, S.; Sasaki, K.; Jee, J.; Kojima, C.; Isogai, A.; Shirakawa, M.. "Structure of the Male Determinant Factor for Brassica Self-incompatibility"  J. Biol. Chem. 278, 36389-36395 (2003).

Assembly members:
S-locus pollen protein, polymer, 50 residues, Formula weight is not available

Natural source:   Common Name: Brassica rapa   Taxonomy ID: 3711   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Brassica rapa

Experimental source:   Production method: Chemically synthetic peptides

Entity Sequences (FASTA):
S-locus pollen protein: NLMKRCTRGFRKLGKCTTLE EEKCKTLYPRGQCTCSDSKM NTHSCDCKSC

Data sets:
Data typeCount
1H chemical shifts282

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1S-locus pollen protein1

Entities:

Entity 1, S-locus pollen protein 50 residues - Formula weight is not available

1   ASNLEUMETLYSARGCYSTHRARGGLYPHE
2   ARGLYSLEUGLYLYSCYSTHRTHRLEUGLU
3   GLUGLULYSCYSLYSTHRLEUTYRPROARG
4   GLYGLNCYSTHRCYSSERASPSERLYSMET
5   ASNTHRHISSERCYSASPCYSLYSSERCYS

Samples:

sample_1: S-locus pollen protein 0.5 mM; phosphate buffer 50 mM; KCl 50 mM; H2O 95%; D2O 5%

sample_2: S-locus pollen protein 0.5 mM; phosphate buffer 50 mM; KCl 50 mM; D2O 99.8%

sample_cond_1: ionic strength: 500 mM; pH: 6.5 na; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available

Software:

CNS v1.1 - refinement

NMR spectrometers:

  • Bruker DRX 800 MHz

Related Database Links:

PDB
DBJ BAA92246 BAF91376
GB AAF17505