BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5849

Title: Solution structure of the PCI domain

Authors: Suzuki, S.; Hatanaka, H.; Kigawa, T.; Shirouzu, M.; Hayashizaki, Y.; Yokoyama, S.

Citation: Suzuki, S.; Hatanaka, H.; Kigawa, T.; Shirouzu, M.; Hayashizaki, Y.; Yokoyama, S.. "Solution structure of the PCI domain"  . ., .-..

Assembly members:
COP9 complex subunit 4, polymer, 84 residues, Formula weight is not available

Natural source:   Common Name: house mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
COP9 complex subunit 4: GSSGSSGGSSILDRAVIEHN LLSASKLYNNITFEELGALL EIPAAKAEKIASQMITEGRM NGFIDQIDGIVHFETREASG PSSG

Data sets:
Data typeCount
1H chemical shifts510
13C chemical shifts338
15N chemical shifts83

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1COP9 complex subunit 41

Entities:

Entity 1, COP9 complex subunit 4 84 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYGLYSERSER
2   ILELEUASPARGALAVALILEGLUHISASN
3   LEULEUSERALASERLYSLEUTYRASNASN
4   ILETHRPHEGLUGLULEUGLYALALEULEU
5   GLUILEPROALAALALYSALAGLULYSILE
6   ALASERGLNMETILETHRGLUGLYARGMET
7   ASNGLYPHEILEASPGLNILEASPGLYILE
8   VALHISPHEGLUTHRARGGLUALASERGLY
9   PROSERSERGLY

Samples:

sample_1: COP9 complex subunit 4, [U-13C; U-15N], 6.1 mM; sodium phosphate buffer 20 mM; NaCl 100 mM; d-DTT 1 mM; dithiothreitol, [U-100% 2H], 1 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 6.0 na; temperature: 298 K; ionic strength: 0.12 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablenot available
3D 13C-separated NOESYnot availablenot availablenot available

Software:

VNMR v6.1B - collection

NMRPipe v20020425 - processing

NMRView v5.0.4 - data analysis

Kujira v0.771 - data analysis

Olivia v1.9.5 - data analysis

CYANA v1.0.7 - structure solution, refinement

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB
EMBL CAJ82695 CDQ76630 CDQ94141
GB AAI23028 AAI31888 ACN11367 AFK11174 ETE57687
REF NP_001016715 NP_001091298 NP_001167326 NP_001279365 XP_004472727