BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5851

Title: Solution Stucture of the Third Zinc Finger from BKLF   PubMed: 12736264

Authors: Simpson, R.; Cram, E.; Czolij, R.; Matthews, J.; Crossley, M.; Mackay, J.

Citation: Simpson, R.; Cram, E.; Czolij, R.; Matthews, J.; Crossley, M.; Mackay, J.. "CCHX Zinc Finger Derivatives Retain the Ability to Bind Zn(II) and Mediate Protein-DNA Interactions"  J. Biol. Chem. 278, 28011-28018 (2003).

Assembly members:
Kruppel-like factor 3, polymer, 37 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Kruppel-like factor 3: GSTRGSTGIKPFQCPDCDRS FSRSDHLALHRKRHMLV

Data sets:
Data typeCount
15N chemical shifts33
1H chemical shifts213
coupling constants28

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Kruppel-like factor 31
2ZINC ION2

Entities:

Entity 1, Kruppel-like factor 3 37 residues - Formula weight is not available

1   GLYSERTHRARGGLYSERTHRGLYILELYS
2   PROPHEGLNCYSPROASPCYSASPARGSER
3   PHESERARGSERASPHISLEUALALEUHIS
4   ARGLYSARGHISMETLEUVAL

Entity 2, ZINC ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: Kruppel-like factor 3 3 mM; TCEP 4.5 mM; ZnSO4 4.5 mM; H2O 90%; D2O 10%

sample_2: Kruppel-like factor 3, [U-15N], 1 mM; TCEP 1.5 mM; ZnSO4 1.5 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 5.5; pressure: 1 atm; temperature: 280 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablesample_cond_1
2D TOCSYnot availablenot availablesample_cond_1
DQF-COSYnot availablenot availablesample_cond_1
HNHAnot availablenot availablesample_cond_1

Software:

xwinnmr v2.5 - processing

DYANA v1.5 -

XEASY v1.3.13 - data analysis

ARIA v1.1.2 - refinement, structure solution

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

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