BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5861

Title: Backbone resonance assignments of GTPase domain of E.coli MnmE   PubMed: 14752268

Authors: Monleon, Daniel; Yim, Lucia; Martinez-Vicente, Marta; Armengod, M.; Celda, Bernardo

Citation: Monleon, Daniel; Yim, Lucia; Martinez-Vicente, Marta; Armengod, M.; Celda, Bernardo. "Letter to the Editor: Backbone 1H, 13C and 15N resonance assignments for the 18.7 kDa GTPase domain of Escherichia coli MnmE protein"  J. Biomol. NMR 28, 307-308 (2004).

Assembly members:
gtpase domain mnme, polymer, 172 residues, Formula weight is not available

Natural source:   Common Name: Escherichia coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: escherichia coli

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
gtpase domain mnme: GSLLREGMKVVIAGRPNAGK SSLLNALAGREAAIVTDIAG TTRDVLREHIHIDGMPLHII DTAGLREASDEVERIGIERA WQEIEQADRVLFMVDGTTTD AVDPAEIWPEFIARLPAKLP ITVVRNKADITGETLGMSEV NGHALIRLSARTGEGVDVLR NHLKQSMGIHRD

Data sets:
Data typeCount
1H chemical shifts329
13C chemical shifts462
15N chemical shifts159

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1gtpase domain MnmE1

Entities:

Entity 1, gtpase domain MnmE 172 residues - Formula weight is not available

1   GLYSERLEULEUARGGLUGLYMETLYSVAL
2   VALILEALAGLYARGPROASNALAGLYLYS
3   SERSERLEULEUASNALALEUALAGLYARG
4   GLUALAALAILEVALTHRASPILEALAGLY
5   THRTHRARGASPVALLEUARGGLUHISILE
6   HISILEASPGLYMETPROLEUHISILEILE
7   ASPTHRALAGLYLEUARGGLUALASERASP
8   GLUVALGLUARGILEGLYILEGLUARGALA
9   TRPGLNGLUILEGLUGLNALAASPARGVAL
10   LEUPHEMETVALASPGLYTHRTHRTHRASP
11   ALAVALASPPROALAGLUILETRPPROGLU
12   PHEILEALAARGLEUPROALALYSLEUPRO
13   ILETHRVALVALARGASNLYSALAASPILE
14   THRGLYGLUTHRLEUGLYMETSERGLUVAL
15   ASNGLYHISALALEUILEARGLEUSERALA
16   ARGTHRGLYGLUGLYVALASPVALLEUARG
17   ASNHISLEULYSGLNSERMETGLYILEHIS
18   ARGASP

Samples:

sample_1: gtpase domain mnme, [U-95% 13C; U-95% 15N], 0.2 – 0.4 mM

sample_cond_1: pH: 6.8; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15N-HSQCnot availablenot availablenot available
HNCOnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available

Software:

Xwinnmr v3.1 - data processing

Sparky v3.104 - spectra analysis, peak picking, manual resonance assignments

AutoAssign v1.9.0 - automatic resonances pre-assignment

NMR spectrometers:

  • AVANCE DRX-AVANCE 500 MHz

Related Database Links:

PDB
DBJ BAB38064 BAE77587 BAG79516 BAI28034 BAI33153
EMBL CAP78166 CAQ34051 CAQ91437 CAR00681 CAR05336
GB AAA62057 AAC76729 AAG58903 AAN45201 AAN83061
REF NP_290339 NP_312668 NP_418162 NP_709494 NP_756487
SP A1AHP0 A7ZTR2 A8A6G8 B1IX32 B1LL33