BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5865

Title: Solution Structure of Bmkk 2, A Novel Potassium Channel Blocker from Scorpion Buthus martensi Karsch   PubMed: 15146482

Authors: Wu, H.; Zhang, N.; Wang, Y.; Zhang, Q.; Ou, L.; Li, M.; Hu, G.

Citation: Zhang, Naixia; Li, Minghua; Chen, Xiang; Wang, Yuefeng; Wu, Gong; Hu, Guoyuan; Wu, Houming. "Solution structure of BmKK2, a new potassium channel blocker from the venom of chinese scorpion Buthus martensi Karsch"  Proteins 55, 835-845 (2004).

Assembly members:
K+ toxin-like peptide, polymer, 31 residues, Formula weight is not available

Natural source:   Common Name: Chinese scorpion   Taxonomy ID: 34649   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Buthus martensii Karsch

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):
K+ toxin-like peptide: TPFAIKCATDADCSRKCPGN PPCRNGFCACT

Data sets:
Data typeCount
1H chemical shifts191

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1K+ toxin-like peptide1

Entities:

Entity 1, K+ toxin-like peptide 31 residues - Formula weight is not available

1   THRPROPHEALAILELYSCYSALATHRASP
2   ALAASPCYSSERARGLYSCYSPROGLYASN
3   PROPROCYSARGASNGLYPHECYSALACYS
4   THR

Samples:

sample_1: K+ toxin-like peptide 2.7 mM; H2O 90%; D2O 10%

sample_2: K+ toxin-like peptide 2.7 mM; D2O 100%

sample_cond_1: pH: 3.0; temperature: 300 K; pressure: 1 atm

sample_cond_2: pH: 2.6; temperature: 300 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
DQF-COSYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available

Software:

VNMR v6.1B - collection, processing

XEASY v1994 - data analysis

DYANA v1.5 - structure solution

AMBER v5.0 - refinement

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
EMBL CAC38036 CAC38039
GB AAK61822 AAQ13566 AFR43620
SP Q95NK7