BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5868

Title: Solution structure of XPC binding domain of hHR23B   PubMed: 15885096

Authors: Kim, B.; Ryu, K.-S.; Kim, H.; Choi, B.-S.

Citation: Kim, B.; Ryu, K.-S.; Kim, H.; Cho, S.; Choi, B.-S.. "Solution structure and backbone dynamics of the XPC-binding domain of the human DNA repair protein hHR23B."  FEBS J. 272, 2467-2476 (2005).

Assembly members:
RAD23 homolog B, polymer, 72 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
RAD23 homolog B: GSHMPLEFLRNQPQFQQMRQ IIQQNPSLLPALLQQIGREN PQLLQQISQHQEHFIQMLNE PVQEAGGQGGGG

Data sets:
Data typeCount
13C chemical shifts269
15N chemical shifts82
1H chemical shifts461

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RAD23 homolog B1

Entities:

Entity 1, RAD23 homolog B 72 residues - Formula weight is not available

1   GLYSERHISMETPROLEUGLUPHELEUARG
2   ASNGLNPROGLNPHEGLNGLNMETARGGLN
3   ILEILEGLNGLNASNPROSERLEULEUPRO
4   ALALEULEUGLNGLNILEGLYARGGLUASN
5   PROGLNLEULEUGLNGLNILESERGLNHIS
6   GLNGLUHISPHEILEGLNMETLEUASNGLU
7   PROVALGLNGLUALAGLYGLYGLNGLYGLY
8   GLYGLY

Samples:

sample_1: RAD23 homolog B, [U-15N; U-13C], 1 mM; phosphate buffer NA 40 mM; CHAPS NA 10 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 210 mM; pH: 7.0; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablenot available
3D 13C-separated NOESYnot availablenot availablenot available
HNHAnot availablenot availablenot available

Software:

VNMR v6.3c - collection

NMRPipe - processing

CYANA v1.6 - structure solution

AMBER v7 - refinement

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAA04652 BAD97199 BAE89144 BAE89405 BAG54170
EMBL CAA63146
GB AAH20973 AAH27747 AAH70960 AAH90351 AAH98674
PRF 2206377B
REF NP_001020446 NP_001039775 NP_001231638 NP_001231642 NP_001231653
SP P54727 Q29RK4 Q4KMA2
TPG DAA26518