BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5873

Title: Backbone and side chains 1H, 13C, and 15N Chemical Shift Assignments for BlaI-NTD   PubMed: 14568532

Authors: Van Melckebeke, Helene; Vreuls, Christelle; Gans, Pierre; Filee, Patrice; Llabres, Gabriel; Joris, Bernard; Simorre, Jean-Pierre

Citation: Van Melckebeke, Helene; Vreuls, Christelle; Gans, Pierre; Filee, Patrice; Llabres, Gabriel; Joris, Bernard; Simorre, Jean-Pierre. "Solution Structural Study of BlaI : Implications for the Repression of Genes Involved in Beta-lactam Antibiotic Resistance"  J. Mol. Biol. 333, 711-720 (2003).

Assembly members:
b-lactamase gene repressor, polymer, 82 residues, Formula weight is not available

Natural source:   Common Name: Bacillus licheniformis   Taxonomy ID: 1402   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Bacillus licheniformis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
b-lactamase gene repressor: MKKIPQISDAELEVMKVIWK HSSINTNEVIKELSKTSTWS PKTIQTMLLRLIKKGALNHH KEGRVFVYTPNIDESDYIEV KS

Data sets:
Data typeCount
1H chemical shifts513
13C chemical shifts351
15N chemical shifts76

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1BlaI-NTD1

Entities:

Entity 1, BlaI-NTD 82 residues - Formula weight is not available

1   METLYSLYSILEPROGLNILESERASPALA
2   GLULEUGLUVALMETLYSVALILETRPLYS
3   HISSERSERILEASNTHRASNGLUVALILE
4   LYSGLULEUSERLYSTHRSERTHRTRPSER
5   PROLYSTHRILEGLNTHRMETLEULEUARG
6   LEUILELYSLYSGLYALALEUASNHISHIS
7   LYSGLUGLYARGVALPHEVALTYRTHRPRO
8   ASNILEASPGLUSERASPTYRILEGLUVAL
9   LYSSER

Samples:

sample_1: b-lactamase gene repressor, [U-13C; U-15N], 0.5 mM; phosphate buffer 75 mM; KCl 300 mM; H2O 90%; D2O 10%

sample_2: b-lactamase gene repressor, [U-15N], 0.75 mM

set_condition_1: pH: 7.6 na; temperature: 298 K; ionic strength: 375 mM

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availablenot available
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
CBCANHnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
(H)C(CO)NH-TOCSYnot availablenot availablenot available
H(CCO)NH-TOCSYnot availablenot availablenot available
1H-15N HSQCnot availablenot availablenot available
H(C)CH-TOCSYnot availablenot availablenot available
2D-NOESYnot availablenot availablenot available

Software:

FELIX v2000 -

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz

Related Database Links:

PDB
GB AAA22648 AAA22650 AAU21886 AAU39238 AGN34711
REF WP_003178582 WP_020449991 WP_026585720 YP_006711714 YP_008076448
SP P06555