BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5874

Title: Complete backbone resonance assignments of p47: the 41kDa adaptor protein of the AAA ATPase p97   PubMed: 14752269

Authors: Yuan, Xuemei; Simpson, Pete; Kondo, Hisao; Mckeown, Ciaran; Dreveny, Ingrid; Shaw, Anthony; Zhang, Xiaodong; Freemont, Paul; Matthews, Stephen

Citation: Yuan, Xuemei; Simpson, Pete; Kondo, Hisao; Mckeown, Ciaran; Dreveny, Ingrid; Shaw, Anthony; Zhang, Xiaodong; Freemont, Paul; Matthews, Stephen. "Complete backbone resonance assignments of p47: the 41kDa adaptor protein of the AAA ATPase p97"  J. Biomol. NMR 28, 309-310 (2004).

Assembly members:
p47, polymer, 174 residues, 41000 Da.

Natural source:   Common Name: Norway rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
p47: MAEERQDALREFVAVTGAEE DRARFFLESAGWDLQIALAS FYEDGGDEDIVTISQATPSS VSRGTAPSDNRVTSFRDLIH DQDEEEEEEEGQRFYAGGSE RSGQQIVGPPRKKSPNELVD DLFKGAKEHGAVAVERVTKS PGETSKPRPFAGGGYRLGAA PEEESAYVAGERRR

Data sets:
Data typeCount
13C chemical shifts282
15N chemical shifts140
1H chemical shifts759

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1p47 residues 1-174, p47 monomer1

Entities:

Entity 1, p47 residues 1-174, p47 monomer 174 residues - 41000 Da.

1   METALAGLUGLUARGGLNASPALALEUARG
2   GLUPHEVALALAVALTHRGLYALAGLUGLU
3   ASPARGALAARGPHEPHELEUGLUSERALA
4   GLYTRPASPLEUGLNILEALALEUALASER
5   PHETYRGLUASPGLYGLYASPGLUASPILE
6   VALTHRILESERGLNALATHRPROSERSER
7   VALSERARGGLYTHRALAPROSERASPASN
8   ARGVALTHRSERPHEARGASPLEUILEHIS
9   ASPGLNASPGLUGLUGLUGLUGLUGLUGLU
10   GLYGLNARGPHETYRALAGLYGLYSERGLU
11   ARGSERGLYGLNGLNILEVALGLYPROPRO
12   ARGLYSLYSSERPROASNGLULEUVALASP
13   ASPLEUPHELYSGLYALALYSGLUHISGLY
14   ALAVALALAVALGLUARGVALTHRLYSSER
15   PROGLYGLUTHRSERLYSPROARGPROPHE
16   ALAGLYGLYGLYTYRARGLEUGLYALAALA
17   PROGLUGLUGLUSERALATYRVALALAGLY
18   GLUARGARGARG

Samples:

sample_1: p47, [U-13C; U-15N], 0.5 mM

ex-cond: pH: 5.3; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCOnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HBHA(CBCACO)NHnot availablenot availablenot available
1H-15N NOESYnot availablenot availablenot available
1H-13C NOESYnot availablenot availablenot available

Software:

NMRPipe -

NMRView -

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Varian . 800 MHz

Related Database Links:

DBJ BAA21659 BAA91731 BAB28604 BAC30250 BAD97061
EMBL CAA71742 CAH90897
GB AAD44488 AAH02801 AAH50936 AAH72464 AAI02957
REF NP_001029729 NP_001125510 NP_001231372 NP_001278003 NP_057227
SP O35987 Q3SZC4 Q5RBG3 Q9CZ44 Q9UNZ2
TPG DAA23261