BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5875

Title: 1H, 13C, and 15N Chemical Shift Assignments of the dimeric mutant of GB1   PubMed: 14516749

Authors: Byeon, In-Ja; Louis, John; Gronenborn, Angela

Citation: Byeon, In-Ja; Louis, John; Gronenborn, Angela. "A Protein Contortionist: Core Mutations of GB1 that Induce Dimerization and Domain Swapping"  J. Mol. Biol. 333, 141-152 (2003).

Assembly members:
Immunoglobulin Binding domain 1 of Protein G, polymer, 56 residues, 6220.8 Da.

Natural source:   Common Name: Streptococcus sp. (Lancefield Group G)   Taxonomy ID: 1306   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Streptococcus Streptococcus sp. (Lancefield Group G)

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Immunoglobulin Binding domain 1 of Protein G: MQYKVILNGKTLKGETTTEA VDAATAEKVVKQFFNDNGVD GEWTYDDATKTFTVTE

Data sets:
Data typeCount
13C chemical shifts175
15N chemical shifts61
1H chemical shifts362

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GB1 subunit A1
2GB1 subunit B1

Entities:

Entity 1, GB1 subunit A 56 residues - 6220.8 Da.

1   METGLNTYRLYSVALILELEUASNGLYLYS
2   THRLEULYSGLYGLUTHRTHRTHRGLUALA
3   VALASPALAALATHRALAGLULYSVALVAL
4   LYSGLNPHEPHEASNASPASNGLYVALASP
5   GLYGLUTRPTHRTYRASPASPALATHRLYS
6   THRPHETHRVALTHRGLU

Samples:

sample_1: Immunoglobulin Binding domain 1 of Protein G, [U-95% 13C; U-95% 15N], 1.7 mM

Ex-cond_1: pH: 6.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYnot availablenot availablenot available
2D 1H-1H TOCSYnot availablenot availablenot available
2D 1H-15N HSQCnot availablenot availablenot available
2D 1H-13C HSQC/HMQCnot availablenot availablenot available
3D HNCOnot availablenot availablenot available
3D HNCACBnot availablenot availablenot available
3D CBCA(CO)NHnot availablenot availablenot available
3D H(CCO)NH-TOCSYnot availablenot availablenot available
3D C(CCO)NH-TOCSYnot availablenot availablenot available
3D HNHBnot availablenot availablenot available
3D HACAHB-COSYnot availablenot availablenot available
2D 13Cnot availablenot availablenot available
5875not availablenot availablenot available
not availablenot availablenot availablenot available
not availableifferencearom'not available
not available62D [13C-13C] long-range correlation experimentnot available
not available73D 15N-edited TOCSYnot available
not available83D 15N-edited NOESYnot available
not availablenot availablenot availablenot available

Software:

NMRPipe v2.2 - processing

PIPP v4.3.2 - data analysis

NMR spectrometers:

  • Bruker DRX 800 MHz
  • Bruker DMX 750 MHz
  • Bruker DRX 600 MHz
  • Bruker DMX 600 MHz
  • Bruker DMX 500 MHz

Related Database Links:

BMRB 5654
PDB