BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5876

Title: Complete backbone resonance assignments of p47: the 41kDa adaptor protein of the AAA ATPase p97   PubMed: 14752269

Authors: Yuan, Xuemei; Simpson, Pete; Kondo, Hisao; Mckeown, Ciaran; Dreveny, Ingrid; Shaw, Anthony; Zhang, Xiaodong; Freemont, Paul; Matthews, Stephen

Citation: Yuan, Xuemei; Simpson, Pete; Kondo, Hisao; Mckeown, Ciaran; Dreveny, Ingrid; Zhang, Xiaodong; Freemont, Paul; Matthews, Stephen. "Letter to the Editor: Complete backbone resonance assignments of p47: the 41kDa adaptor protein of the AAA ATPase p97"  J. Biomol. NMR 28, 309-310 (2004).

Assembly members:
p47, polymer, 200 residues, Formula weight is not available

Natural source:   Common Name: Norway rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
p47: ERRRHSGQDVHVVLKLWKTG FSLDNGDLRSYQDPSNAQFL ESIRRGEVPAELRRLAHGGQ VNLDMEDHRDEDFVKPKGAF KAFTGEGQKLGSTAPQVLNT SSPAQQAENEAKASSSILIN EAEPTTNIQIRLADGGRLVQ KFNHSHRISDIRLFIVDARP AMAATSFVLMTTFPNKELAD ENQTLKEANLLNAVIVQRLT

Data sets:
Data typeCount
1H chemical shifts1205
13C chemical shifts563
15N chemical shifts182

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1p47 residues 171-370, p47 monomer1

Entities:

Entity 1, p47 residues 171-370, p47 monomer 200 residues - Formula weight is not available

1   GLUARGARGARGHISSERGLYGLNASPVAL
2   HISVALVALLEULYSLEUTRPLYSTHRGLY
3   PHESERLEUASPASNGLYASPLEUARGSER
4   TYRGLNASPPROSERASNALAGLNPHELEU
5   GLUSERILEARGARGGLYGLUVALPROALA
6   GLULEUARGARGLEUALAHISGLYGLYGLN
7   VALASNLEUASPMETGLUASPHISARGASP
8   GLUASPPHEVALLYSPROLYSGLYALAPHE
9   LYSALAPHETHRGLYGLUGLYGLNLYSLEU
10   GLYSERTHRALAPROGLNVALLEUASNTHR
11   SERSERPROALAGLNGLNALAGLUASNGLU
12   ALALYSALASERSERSERILELEUILEASN
13   GLUALAGLUPROTHRTHRASNILEGLNILE
14   ARGLEUALAASPGLYGLYARGLEUVALGLN
15   LYSPHEASNHISSERHISARGILESERASP
16   ILEARGLEUPHEILEVALASPALAARGPRO
17   ALAMETALAALATHRSERPHEVALLEUMET
18   THRTHRPHEPROASNLYSGLULEUALAASP
19   GLUASNGLNTHRLEULYSGLUALAASNLEU
20   LEUASNALAVALILEVALGLNARGLEUTHR

Samples:

sample_1: p47, [U-13C; U-15N], 0.5 mM

ex-cond: pH: 5.3; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCOnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HBHA(CBCACO)NHnot availablenot availablenot available
1H-15N NOESYnot availablenot availablenot available
1H-13C NOESYnot availablenot availablenot available

Software:

NMRPipe -

NMRView -

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Varian . 800 MHz

Related Database Links:

PDB
DBJ BAA21659 BAB28604 BAE23234
EMBL CAA71742
GB AAH50936 AAH72464 AAI02957 AAI06102 EDL05910
REF NP_001029729 NP_001278003 NP_114187 NP_938085 XP_003411671
SP O35987 Q3SZC4 Q9CZ44
TPG DAA23261