BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5879

Title: Structure of a Type IVb pilin from Salmonella typhi and its assembly into pilus   PubMed: 15159389

Authors: Xu, Xing-Fu; Tan, Yih-Wan; Hackett, James; Zhang, Mingjie; Mok, Yu-Keung

Citation: Xu, Xing-Fu; Tan, Yih-Wan; Lam, L.; Hackett, James; Zhang, Mingjie; Mok, Yu-Keung. "NMR Structure of a Type IVb pilin from Salmonella typhi and its assembly into pilus"  J. Biol. Chem. ., .-. (2004).

Assembly members:
S. typhi PilS, polymer, 156 residues, Formula weight is not available

Natural source:   Common Name: Salmonella typhi   Taxonomy ID: 601   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Salmonella typhi

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
S. typhi PilS: MWGKKDAGTELTNYQTLATN TIGMMKGVDGYAFTSGAKMT DTLIQAGAAKGMTVSGDPAS GSATLWNSWGGQIVVAPDTA GGTGFNNGFTITTNKVPQSA CVSISTGMSRSGGTSGIKIN GNNHTDAKVTAEIASSECTA DNGRTGTNTLVFNYNG

Data sets:
Data typeCount
1H chemical shifts883
13C chemical shifts563
15N chemical shifts152

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1S.typhi type IVb pilin1

Entities:

Entity 1, S.typhi type IVb pilin 156 residues - Formula weight is not available

1   METTRPGLYLYSLYSASPALAGLYTHRGLU
2   LEUTHRASNTYRGLNTHRLEUALATHRASN
3   THRILEGLYMETMETLYSGLYVALASPGLY
4   TYRALAPHETHRSERGLYALALYSMETTHR
5   ASPTHRLEUILEGLNALAGLYALAALALYS
6   GLYMETTHRVALSERGLYASPPROALASER
7   GLYSERALATHRLEUTRPASNSERTRPGLY
8   GLYGLNILEVALVALALAPROASPTHRALA
9   GLYGLYTHRGLYPHEASNASNGLYPHETHR
10   ILETHRTHRASNLYSVALPROGLNSERALA
11   CYSVALSERILESERTHRGLYMETSERARG
12   SERGLYGLYTHRSERGLYILELYSILEASN
13   GLYASNASNHISTHRASPALALYSVALTHR
14   ALAGLUILEALASERSERGLUCYSTHRALA
15   ASPASNGLYARGTHRGLYTHRASNTHRLEU
16   VALPHEASNTYRASNGLY

Samples:

sample_1: S. typhi PilS, [U-99% 13C; U-99% 15N], 1.0 mM; phosphate buffer 50 mM; Na2SO4 0.5 M

sample_2: S. typhi PilS, [U-99% 15N], 1.0 mM; phosphate buffer 50 mM; Na2SO4 0.5 M

sample_3: S. typhi PilS, [U-10% 13C], 1.0 mM; phosphate buffer 50 mM; Na2SO4 0.5 M

Ex-cond_1: pH: 6.0 na; temperature: 303 K; ionic strength: 0.5 M

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
CC(C0)NHnot availablenot availablenot available
H(CCO)NHnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
1H-13C CT HSQCnot availablenot availablenot available

Software:

NMRPipe -

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Varian Inova 750 MHz

Related Database Links:

PDB
EMBL CAD09325 CAX67827 CBJ94782
GB AAC98887 AAF81215 AAO71706 AAP83037 AEZ48065
PIR AD1028
REF NP_458635 NP_807846 WP_000319138 WP_001271533 WP_010989286