BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5880

Title: Solution structure of the retroviral Gag MA-like domain of RIKEN cDNA 3110009E22

Authors: Suzuki, S.; Hatanaka, H.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Hayashizaki, Y.; Yokoyama, S.

Citation: Suzuki, S.; Hatanaka, H.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Hayashizaki, Y.; Yokoyama, S.. "Solution structure of the retroviral Gag MA-like domain of RIKEN cDNA 3110009E22"  . ., .-..

Assembly members:
RIKEN cDNA 3110009E22, polymer, 105 residues, Formula weight is not available

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
RIKEN cDNA 3110009E22: GSSGSSGTPLSLTLDHWSEI RSRAHNLSVEIKKGPWRTFC ASEWPTFDVGWPPEGTFDLT VIFEVKAIVFQDGPGSHPDQ QPYITVWQDLVQNSPPWIKS GPSSG

Data sets:
Data typeCount
13C chemical shifts452
15N chemical shifts108
1H chemical shifts675

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1product of RIKEN cDNA 3110009E221

Entities:

Entity 1, product of RIKEN cDNA 3110009E22 105 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYTHRPROLEU
2   SERLEUTHRLEUASPHISTRPSERGLUILE
3   ARGSERARGALAHISASNLEUSERVALGLU
4   ILELYSLYSGLYPROTRPARGTHRPHECYS
5   ALASERGLUTRPPROTHRPHEASPVALGLY
6   TRPPROPROGLUGLYTHRPHEASPLEUTHR
7   VALILEPHEGLUVALLYSALAILEVALPHE
8   GLNASPGLYPROGLYSERHISPROASPGLN
9   GLNPROTYRILETHRVALTRPGLNASPLEU
10   VALGLNASNSERPROPROTRPILELYSSER
11   GLYPROSERSERGLY

Samples:

sample_1: RIKEN cDNA 3110009E22, [U-13C; U-15N], 3.2 mM; phosphate buffer Na 20 mM; NaCl 100 mM; d-DTT 1 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 0.12 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablenot available
3D 13C-separated NOESYnot availablenot availablenot available

Software:

VNMR v6.1B - collection

NMRPipe v20020425 - processing

NMRView v5.0.4 - data analysis

Kujira v0.771 - data analysis

Olivia v1.9.5 - data analysis

CYANA v1.0.7 - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

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