BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5885

Title: CHEMICAL SHIFTS OF A FUSED DOCKING DOMAIN FROM THE ERYTHROMYCIN POLYKETIDE SYNTHASE (DEBS), A MODEL FOR THE INTERACTION BETWEEN DEBS 2 AND DEBS 3   PubMed: 12954331

Authors: Broadhurst, Richard; Nietlispach, Daniel; Wheatcroft, Michael; Leadlay, Peter; Weissman, Kira

Citation: Broadhurst, Richard; Nietlispach, Daniel; Wheatcroft, Michael; Leadlay, Peter; Weissman, Kira. "The Structure of Docking Domains in Modular Polyketide Synthases"  Chem. Biol. 10, 723-731 (2003).

Assembly members:
6-deoxyerythronolide B synthase, polymer, 120 residues, Formula weight is not available

Natural source:   Common Name: Streptomyces erythraeus   Taxonomy ID: 1836   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Saccharopolyspora erythraea

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
6-deoxyerythronolide B synthase: GSAASPAVDIGDRLDELEKA LEALSAEDGHDDVGQRLESL LRRWNSRRADAPSTSAISED ASDDELFSMLDQRFGGGEDL LMSGDNGMTEEKLRRYLKRT VTELDSVTARLREVEHRAGE

Data sets:
Data typeCount
1H chemical shifts672
13C chemical shifts391
15N chemical shifts116

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DOCK23 subunit 11
2DOCK23 subunit 21

Entities:

Entity 1, DOCK23 subunit 1 120 residues - Formula weight is not available

1   GLYSERALAALASERPROALAVALASPILE
2   GLYASPARGLEUASPGLULEUGLULYSALA
3   LEUGLUALALEUSERALAGLUASPGLYHIS
4   ASPASPVALGLYGLNARGLEUGLUSERLEU
5   LEUARGARGTRPASNSERARGARGALAASP
6   ALAPROSERTHRSERALAILESERGLUASP
7   ALASERASPASPGLULEUPHESERMETLEU
8   ASPGLNARGPHEGLYGLYGLYGLUASPLEU
9   LEUMETSERGLYASPASNGLYMETTHRGLU
10   GLULYSLEUARGARGTYRLEULYSARGTHR
11   VALTHRGLULEUASPSERVALTHRALAARG
12   LEUARGGLUVALGLUHISARGALAGLYGLU

Samples:

sample_1: 6-deoxyerythronolide B synthase, [U-100% 13C; U-100% 15N], 1.0 mM; NaH2PO4 100 mM

sample_2: 6-deoxyerythronolide B synthase, [100% 13C; 100% 15N], 0.5 mM; 6-deoxyerythronolide B synthase 0.5 mM

condition_set_1: pH: 6.5 na; temperature: 298 K; ionic strength: 0.10 mM

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCnot availablenot availablenot available
2D 1H-13C HSQCnot availablenot availablenot available
3D HNCACBnot availablenot availablenot available
3D CBCA(CO)NHnot availablenot availablenot available
3D HBHA(CBCACO)NHnot availablenot availablenot available
3D HNCAnot availablenot availablenot available
3D HN(CO)CAnot availablenot availablenot available
3D HNCOnot availablenot availablenot available
3D 15N-SEPARATED NOESYnot availablenot availablenot available
3D 15N-SEPARATED TOCSYnot availablenot availablenot available
3D 13C-SEPARATED NOESYnot availablenot availablenot available
3D HCCH-TOCSYnot availablenot availablenot available
3D 13C/15N X-FILTERED 13C-SEPARATED NOESYnot availablenot availablenot available

Software:

AZARA v2.7 - DATA PROCESSING

ANSIG v3.3 - MANUAL ASSIGNMENT

ANSIG v3.3 - MANUAL ASSIGNMENT

NMR spectrometers:

  • BRUKER DRX 500 MHz
  • BRUKER DRX 800 MHz

Related Database Links:

PDB
EMBL CAA44448 CAM00064
GB AAA26494 AAV39551 AAV51821 EQD87035
REF WP_011873138 WP_037306412 YP_001102990
SP Q03132