BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5891

Title: 1H, 15N and 13C NMR Assignments and Secondary Structure of a Hyperthermophile DNA-binding Protein Predicted to contain an Extended Coiled Coil Domain

Authors: Kahsai, Mebrahtu; Singh, Sanjay; Gupta, Ramesh; Edmondson, Stephen; Shriver, John

Citation: Kahsai, Mebrahtu; Singh, Sanjay; Gupta, Ramesh; Edmondson, Stephen; Shriver, John. "1H, 15N and 13C NMR Assignments and Secondary Structure of a Hyperthermophile DNA-binding Protein Predicted to contain an Extended Coiled Coil Domain"  J. Biomol. NMR ., .-. (2003).

Assembly members:
Sso10a, polymer, 95 residues, Formula weight is not available

Natural source:   Common Name: Sulfolobus solfataricus   Taxonomy ID: 2287   Superkingdom: Archaea   Kingdom: not available   Genus/species: Sulfolobus solfataricus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Sso10a: AKKKSKLEIIQAILEACKSG SPKTRIMYGANLSYALTGRY IKMLMDLEIIRQEGKQYMLT KKGEELLEDIRKFNEMRKNM DQLKEKINSVLSIRQ

Data sets:
Data typeCount
1H chemical shifts644
13C chemical shifts443
15N chemical shifts93

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Sso10a subunit 11
2Sso10a subunit 21

Entities:

Entity 1, Sso10a subunit 1 95 residues - Formula weight is not available

1   ALALYSLYSLYSSERLYSLEUGLUILEILE
2   GLNALAILELEUGLUALACYSLYSSERGLY
3   SERPROLYSTHRARGILEMETTYRGLYALA
4   ASNLEUSERTYRALALEUTHRGLYARGTYR
5   ILELYSMETLEUMETASPLEUGLUILEILE
6   ARGGLNGLUGLYLYSGLNTYRMETLEUTHR
7   LYSLYSGLYGLUGLULEULEUGLUASPILE
8   ARGLYSPHEASNGLUMETARGLYSASNMET
9   ASPGLNLEULYSGLULYSILEASNSERVAL
10   LEUSERILEARGGLN

Samples:

sample_1: Sso10a, [U-98% 13C; U-98% 15N], 2.5 mM

Condition_1: pH: 5.0 na; temperature: 303 K; ionic strength: 0.0 M

Experiments:

NameSampleSample stateSample conditions
HNHAnot availablenot availablenot available
HNCOnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NNHnot availablenot availablenot available
NOESY-NHSQCnot availablenot availablenot available
CCC-TOCSY-NNHnot availablenot availablenot available
HCC-TOCY-NNHnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
HCCH-COSYnot availablenot availablenot available
HBCBCGCDHDnot availablenot availablenot available
2D-NOESYnot availablenot availablenot available
2D-dq-COSYnot availablenot availablenot available

Software:

NMRVIEW v5 - Back bone and side chain assignment

NMPIPE - NMR data processing

FELIX - NMR data processing

VNMR - data collection, data processing

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB
EMBL CAH69222
GB ACX90575
REF WP_009988494 YP_005642176