BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5892

Title: 1H, 13C and 15N NMR Assignments of the Hyperthermophile Sulfolobus Protein Sso10b2 and its Predicted Secondary Structure   PubMed: 16245938

Authors: Biyani, Kalpesh; Kahsai, Mebrahtu; Edmondson, Stephen; Shriver, John

Citation: Biyani, Kalpesh; Kahsai, Mebrahtu; Clark, Andrew; Armstrong, Tracy; Edmondson, Stephen; Shriver, John. "Solution structure, stability, and nucleic acid binding of the hyperthermophile protein Sso10b2"  Biochemistry 44, 14217-14230 (2005).

Assembly members:
Sso10b2, polymer, 89 residues, Formula weight is not available

Natural source:   Common Name: Sulfolobus solfataricus   Taxonomy ID: 2287   Superkingdom: Archaea   Kingdom: not available   Genus/species: Sulfolobus solfataricus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Sso10b2: MTEKLNEIVVRKTKNVEDHV LDVIVLFNQGIDEVILKGTG REISKAVDVYNSLKDRLGDG VQLVNVQTGSEVRDRRRISY ILLRLKRVY

Data sets:
Data typeCount
1H chemical shifts617
13C chemical shifts400
15N chemical shifts87

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Sso10b2 subunit 11
2Sso10b2 subunit 21

Entities:

Entity 1, Sso10b2 subunit 1 89 residues - Formula weight is not available

1   METTHRGLULYSLEUASNGLUILEVALVAL
2   ARGLYSTHRLYSASNVALGLUASPHISVAL
3   LEUASPVALILEVALLEUPHEASNGLNGLY
4   ILEASPGLUVALILELEULYSGLYTHRGLY
5   ARGGLUILESERLYSALAVALASPVALTYR
6   ASNSERLEULYSASPARGLEUGLYASPGLY
7   VALGLNLEUVALASNVALGLNTHRGLYSER
8   GLUVALARGASPARGARGARGILESERTYR
9   ILELEULEUARGLEULYSARGVALTYR

Samples:

sample_1: Sso10b2, [U-98% 13C; U-98% 15N], 1.0 mM

Condition_1: pH: 4.6; temperature: 303 K; ionic strength: 0.0 M

Experiments:

NameSampleSample stateSample conditions

Software:

Varian -

NMRView -

NMRPipe -

Felix -

NMR spectrometers:

  • VARIAN INOVA 800 MHz

Related Database Links:

PDB
EMBL CAA67067
GB AAK41238 ACP35355 ACP38015 ACP45511 ACP48691
REF NP_342448 WP_009992410 WP_012711268 YP_002829313 YP_002832000
SP P74762 Q97ZF4