BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5899

Title: NMR Solution Structure of BeF3-Activated Spo0F, 20 conformers   PubMed: 12875849

Authors: Gardino, A.; Volkman, B.; Cho, H.; Lee, S.; Wemmer, D.; Kern, D.

Citation: Gardino, A.; Volkman, B.; Cho, H.; Lee, S.; Wemmer, D.; Kern, D.. "The NMR Solution Structure of BeF(3)(-)-activated Spo0F Reveals the Conformational Switch in a Phosphorelay System"  J. Mol. Biol. 331, 245-254 (2003).

Assembly members:
Sporulation initiation phosphotransferase F, polymer, 124 residues, Formula weight is not available

Natural source:   Common Name: Bacillus globigii   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Sporulation initiation phosphotransferase F: MMNEKILIVDDQYGIRILLN EVFNKEGYQTFQAANGLQAL DIVTKERPDLVLLDMKIPGM DGIEILKRMKVIDENIRVII MTAYGELDMIQESKELGALT HFAKPFDIDEIRDAVKKYLP LKSN

Data sets:
Data typeCount
1H chemical shifts826
13C chemical shifts413
15N chemical shifts131

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Neurotoxin BmK371

Entities:

Entity 1, Neurotoxin BmK37 124 residues - Formula weight is not available

1   METMETASNGLULYSILELEUILEVALASP
2   ASPGLNTYRGLYILEARGILELEULEUASN
3   GLUVALPHEASNLYSGLUGLYTYRGLNTHR
4   PHEGLNALAALAASNGLYLEUGLNALALEU
5   ASPILEVALTHRLYSGLUARGPROASPLEU
6   VALLEULEUASPMETLYSILEPROGLYMET
7   ASPGLYILEGLUILELEULYSARGMETLYS
8   VALILEASPGLUASNILEARGVALILEILE
9   METTHRALATYRGLYGLULEUASPMETILE
10   GLNGLUSERLYSGLULEUGLYALALEUTHR
11   HISPHEALALYSPROPHEASPILEASPGLU
12   ILEARGASPALAVALLYSLYSTYRLEUPRO
13   LEULYSSERASN

Samples:

sample_1: Sporulation initiation phosphotransferase F, [U-15N; U-13C], 1 mM; HEPES 20 mM; MgCl2 50 mM; BeCl2 6 mM; NaF 60 mM; D2O 10%; H2O 90%

sample_cond_1: pH: 6.85 na; temperature: 298 K; ionic strength: 116 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available

Software:

DYANA v1.5 - structure solution, refinement

XEASY v1.3.12 - data analysis

NMR spectrometers:

  • Bruker DMX 500 MHz
  • Bruker DMX 600 MHz
  • Bruker DMX 750 MHz

Related Database Links:

BMRB 15008 15009 15010 15011
PDB
DBJ BAI87374 BAM55796 BAM59808 GAK81027
EMBL CAA27217 CAA89872 CAB15730 CCU60811 CEI59521
GB AAA16802 AAA22787 ADM39703 ADP34224 ADV94523
PRF 1306301A
REF NP_391594 WP_003221952 WP_003227621 WP_003326524 WP_010328945
SP P06628