BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5901

Title: Solution Strucure of a CCHHC Domain of Neural Zinc Finger Factor-1   PubMed: 14744132

Authors: Berkovits-Cymet, H.; Amann, B.; Berg, J.

Citation: Berkovits-Cymet, H.; Amann, B.; Berg, J.. "Solution Structure of a CCHHC Domain of Neural Zinc Finger Factor-1 and its Implications for DNA Binding "  Biochemistry 43, 898-903 (2004).

Assembly members:
neural zinc finger transcription factor 1, polymer, 63 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: 10116   Taxonomy ID: Norway rat   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
neural zinc finger transcription factor 1: MHVKKPYYDPSRTEKRESKC PTPGCDGTGHVTGLYPHHRS LSGCPHKDRVPPEILAMHEN VLK

Data sets:
Data typeCount
1H chemical shifts216
15N chemical shifts40

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ZINC BINDING DOMAIN1
2ZINC (II) ION2

Entities:

Entity 1, ZINC BINDING DOMAIN 63 residues - Formula weight is not available

1   METHISVALLYSLYSPROTYRTYRASPPRO
2   SERARGTHRGLULYSARGGLUSERLYSCYS
3   PROTHRPROGLYCYSASPGLYTHRGLYHIS
4   VALTHRGLYLEUTYRPROHISHISARGSER
5   LEUSERGLYCYSPROHISLYSASPARGVAL
6   PROPROGLUILELEUALAMETHISGLUASN
7   VALLEULYS

Entity 2, ZINC (II) ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: neural zinc finger transcription factor 1 2.3 mM; ZnCl2 2.3 mM; deuterated Tris 55 mM

sample_2: neural zinc finger transcription factor 1, [U-15N], 2.8 mM; ZnCl2 2.8 mM; deuterated Tris 36 mM; H2O 98%; D2O 2%

sample_3: neural zinc finger transcription factor 1, [U-15N], 1.5 mM; CdCl2 1.5 mM; deuterated Tris 36 mM

sample_cond_1: pH: 6.95; temperature: 293 K; pressure: 1 atm

sample_cond_2: pH: 7.02; temperature: 303 K; pressure: 1 atm

sample_cond_3: pH: 7.00; temperature: 303 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
DQF-COSYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
15N HSQCnot availablenot availablenot available
15N HSQC LongRangenot availablenot availablenot available
3D 15N HSQC-TOCSYnot availablenot availablenot available
3D 15N HSQC-NOESYnot availablenot availablenot available

Software:

VNMR v6.1b - collection

FELIX v98 - processing

In house perl scripts - data analysis

CNS v1.1 - structure solution, refinement

NMR spectrometers:

  • Varian UNITYplus 500 MHz

Related Database Links:

PDB
DBJ BAC34010