BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5902

Title: 1H, 13C, and 15N Resonance Assignments of the Hath-Domain of Hepatoma-derived Growth Factor   PubMed: 15017145

Authors: Sue, Shih-Che; Huang, Tai-huang

Citation: Sue, Shih-Che; Chen, Jeou-Yuan; Huang, Tai-huang. "Letter to Editor: Sequence specific 1H, 13C and 15N Resonance Assignments of the Hath-Domain of Human Hepatoma-Derived Growth Factor "  J. Biomol. NMR 29, 95-96 (2004).

Assembly members:
hath domain of hHDGF, polymer, 110 residues, 12772.5 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
hath domain of hHDGF: MHHHHHHAMAMSRSNRQKEY KCGDLVFAKMKGYPHWPARI DEMPEAAVKSTANKYQVFFF GTHETAFLGPKDLFPYEESK EKFGKPNKRKGFSEGLWEIE NNPTVKASGY

Data sets:
Data typeCount
1H chemical shifts683
13C chemical shifts455
15N chemical shifts102

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1hath domain1

Entities:

Entity 1, hath domain 110 residues - 12772.5 Da.

1   METHISHISHISHISHISHISALAMETALA
2   METSERARGSERASNARGGLNLYSGLUTYR
3   LYSCYSGLYASPLEUVALPHEALALYSMET
4   LYSGLYTYRPROHISTRPPROALAARGILE
5   ASPGLUMETPROGLUALAALAVALLYSSER
6   THRALAASNLYSTYRGLNVALPHEPHEPHE
7   GLYTHRHISGLUTHRALAPHELEUGLYPRO
8   LYSASPLEUPHEPROTYRGLUGLUSERLYS
9   GLULYSPHEGLYLYSPROASNLYSARGLYS
10   GLYPHESERGLUGLYLEUTRPGLUILEGLU
11   ASNASNPROTHRVALLYSALASERGLYTYR

Samples:

sample_1: hath domain of hHDGF, [U-13C; U-15N], 2.0 mM; Na2HPO4 100 mM; NaCl 150 mM

sample_2: hath domain of hHDGF, [U-13C; U-15N], 1.0 mM; Na2HPO4 100 mM; NaCl 150 mM; D2O 100%

EX-condition: pH: 6.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availableEX-condition
HNCAnot availablenot availableEX-condition
HN(CO)CAnot availablenot availableEX-condition
HNCOnot availablenot availableEX-condition
HN(CA)COnot availablenot availableEX-condition
CBCANHnot availablenot availableEX-condition
CBCA(CO)NHnot availablenot availableEX-condition
HBHA(CO)NHnot availablenot availableEX-condition
1H-15N TOCSYnot availablenot availableEX-condition
H(CC)(CO)NHnot availablenot availableEX-condition
CC(CO)NHnot availablenot availableEX-condition
HCCH-TOCSYnot availablenot availableEX-condition

Software:

XWINNMR v3.1 - NMR data processing

Aurelia v2.8 - NMR data processing, peak picking

NMR spectrometers:

  • Bruker AVANCE 600 MHz
  • Bruker AVANCE 500 MHz

Related Database Links:

PDB
DBJ BAA03903 BAA09838 BAB30979 BAC26466 BAC36126
EMBL CAB40626 CAH92417
GB AAF65469 AAH05713 AAH18991 AAH21654 AAH70943
REF NP_001119522 NP_001119523 NP_004485 NP_032257 NP_446159
SP P51858 P51859 Q8VHK7 Q9XSK7
TPG DAA31759