BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5908

Title: Backbone 1HN and 15N Chemical Shift Assignments for Sac7d V30I

Authors: Clark, Andrew; McCrary, Bradford; Edmondson, Stephen; Shriver, John

Citation: Clark, Andrew; McCrary, Bradford; Edmondson, Stephen; Shriver, John. "Thermodynamics of Hydrophobic Core Packing in the Thermophile Proteins Sac7d and Sso7d"  J. Mol. Biol. ., .-..

Assembly members:
Sac7d, polymer, 66 residues, 7623 Da.

Natural source:   Common Name: Sulfolobus acidocaldarius Brock et al.   Taxonomy ID: 2285   Superkingdom: Archaea   Kingdom: not available   Genus/species: Sulfolobus acidocaldarius

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Sac7d: MVKVKFKYKGEEKEVDTSKI KKVWRVGKMISFTYDDNGKT GRGAVSEKDAPKELLDMLAR AEREKK

Data sets:
Data typeCount
1H chemical shifts67
15N chemical shifts66

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Sac7d V30I subunit 11

Entities:

Entity 1, Sac7d V30I subunit 1 66 residues - 7623 Da.

1   METVALLYSVALLYSPHELYSTYRLYSGLY
2   GLUGLULYSGLUVALASPTHRSERLYSILE
3   LYSLYSVALTRPARGVALGLYLYSMETILE
4   SERPHETHRTYRASPASPASNGLYLYSTHR
5   GLYARGGLYALAVALSERGLULYSASPALA
6   PROLYSGLULEULEUASPMETLEUALAARG
7   ALAGLUARGGLULYSLYS

Samples:

Sac7d_V30I: Sac7d, [U-98% 15N], 1.18 mM; KCl 0.3 M; H2O 90%; D2O 10%; DSS 100 uM

sample_conds: pH: 4.06; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H,15N HSQCSac7d_V30Inot availablesample_conds
3D HSQC-TOCSYSac7d_V30Inot availablesample_conds
3D HSQC-NOESYSac7d_V30Inot availablesample_conds

Software:

NMRPipe - data processing, transformation, referencing

NMRView v5 - sequential assignments

NMR spectrometers:

  • Varian VXR-S 500 MHz
  • Varian INOVA 500 MHz
  • Varian INOVA 800 MHz

Related Database Links:

BMRB 5905
PDB
GB AAA80315 AAY79492 AGE70041 AGE72316 AHC50588
REF WP_011276993 YP_007433189 YP_007435464 YP_254785
SP P13123