BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5909

Title: Backbone 1HN and 15N Chemical Shift Assignments for Sso7d

Authors: Clark, Andrew; McCrary, Bradford; Edmondson, Stephen; Shriver, John

Citation: Clark, Andrew; McCrary, Bradford; Edmondson, Stephen; Shriver, John. "Thermodynamics of Hydrophobic Core Packing in the Thermophile Proteins Sac7d and Sso7d"  J. Mol. Biol. ., .-..

Assembly members:
Sso7d, polymer, 64 residues, 7279 Da.

Natural source:   Common Name: Sulfolobus solfataricus Brock et al   Taxonomy ID: 2285   Superkingdom: Archaea   Kingdom: not available   Genus/species: Sulfolobus solfataricus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Sso7d: MATVKFKYKGEEKQVDISKI KKVWRVGKMISFTYDEGGGK TGRGAVSEKDAPKELLQMLE KQKK

Data sets:
Data typeCount
1H chemical shifts62
13C chemical shifts10
15N chemical shifts62

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Sso7d subunit 11

Entities:

Entity 1, Sso7d subunit 1 64 residues - 7279 Da.

1   METALATHRVALLYSPHELYSTYRLYSGLY
2   GLUGLULYSGLNVALASPILESERLYSILE
3   LYSLYSVALTRPARGVALGLYLYSMETILE
4   SERPHETHRTYRASPGLUGLYGLYGLYLYS
5   THRGLYARGGLYALAVALSERGLULYSASP
6   ALAPROLYSGLULEULEUGLNMETLEUGLU
7   LYSGLNLYSLYS

Samples:

Sso7d_sample_1: Sso7d, [U-99% 13C; U-90% 15N], 0.58 mM; H2O 90%; D2O 10%; DSS 100 uM

pH5: pH: 5.15 na; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H,15N HSQCSso7d_sample_1not availablepH5
HNCACBSso7d_sample_1not availablepH5
CBCA(CO)NNHSso7d_sample_1not availablepH5

Software:

NMRpipe - data processing, transformation, referencing

NMRView v5 - sequential assignments

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 800 MHz

Related Database Links:

BMRB 4570 5910
PDB
DBJ BAA28274
GB AAB25172 AAK42090 AAK42212 AAK42679 ACP34956
PRF 1908252A 2107210A
REF NP_343300 NP_343422 NP_343889 WP_009990119 WP_009992021
SP P39476 P61990 P61991