BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5910

Title: Backbone 1HN and 15N Chemical Shift Assignments for Sso7d I30V   PubMed: 15005619

Authors: Clark, Andrew; McCrary, Bradford; Edmondson, Stephen; Shriver, John

Citation: Clark, Andrew; McCrary, Bradford; Edmondson, Stephen; Shriver, John. "Thermodynamics of core hydrophobicity and packing in the hyperthermophile proteins Sac7d and Sso7d."  Biochemistry 43, 2840-2853 (2004).

Assembly members:
Sso7d, polymer, 64 residues, 7265 Da.

Natural source:   Common Name: Sulfolobus acidocaldarius Brock et al   Taxonomy ID: 2285   Superkingdom: Archaea   Kingdom: not available   Genus/species: Sulfolobus acidocaldarius

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Sso7d: MATVKFKYKGEEKQVDISKI KKVWRVGKMVSFTYDEGGGK TGRGAVSEKDAPKELLQMLE KQKK

Data sets:
Data typeCount
15N chemical shifts62
1H chemical shifts62

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Sso7d monomer1

Entities:

Entity 1, Sso7d monomer 64 residues - 7265 Da.

1   METALATHRVALLYSPHELYSTYRLYSGLY
2   GLUGLULYSGLNVALASPILESERLYSILE
3   LYSLYSVALTRPARGVALGLYLYSMETVAL
4   SERPHETHRTYRASPGLUGLYGLYGLYLYS
5   THRGLYARGGLYALAVALSERGLULYSASP
6   ALAPROLYSGLULEULEUGLNMETLEUGLU
7   LYSGLNLYSLYS

Samples:

I30V_sample_1: Sso7d, [U-98% 15N], 1.28 mM; H2O 90%; D2O 10%; DSS 100 uM

pH5_0salt: pH: 5.07 na; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H,15N HSQCI30V_sample_1not availablepH5_0salt
3D HSQC-TOCSYI30V_sample_1not availablepH5_0salt
3D HSQC-NOESYI30V_sample_1not availablepH5_0salt

Software:

NMRPipe - data processing, referencing, transformation

NMRView v5 - sequential assignments

NMR spectrometers:

  • Varian VXR-S 500 MHz
  • Varian INOVA 800 MHz

Related Database Links:

BMRB 5909
PDB
DBJ BAA28274
GB AAB25172 AAK42090 AAK42212 ACP37540 ACP45250
PRF 1908252A
REF NP_343300 NP_343422 WP_009992021 YP_002828838 YP_002837172
SP P61990 P61991