BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5911

Title: NMR Solution Structure of a Peptide from the mdm-2 Binding Domain of the p53 Protein that is Selectively Cytotoxic to Cancer Cells   PubMed: 14967026

Authors: Rosal, R.; Pincus, M.; Brandt-Rauf, P.; Fine, R.; Wang, H.

Citation: Rosal, R.; Pincus, M.; Brandt-Rauf, P.; Fine, R.; Michl, J.; Wang, H.. "NMR Solution Structure of a Peptide from the mdm-2 Binding Domain of the p53 Protein that is Selectively Cytotoxic to Cancer Cells"  Biochemistry 43, 1854-1861 (2004).

Assembly members:
PNC27, polymer, 32 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
PNC27: PPLSQETFSDLWKLLKKWKM RRNQFWVKVQRG

Data sets:
Data typeCount
13C chemical shifts112
1H chemical shifts228

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PNC271

Entities:

Entity 1, PNC27 32 residues - Formula weight is not available

1   PROPROLEUSERGLNGLUTHRPHESERASP
2   LEUTRPLYSLEULEULYSLYSTRPLYSMET
3   ARGARGASNGLNPHETRPVALLYSVALGLN
4   ARGGLY

Samples:

sample_1: PNC27 4 mM; DMSO-d6 5%; sodium phosphate 10 mM; H2O 95%

sample_cond_1: ionic strength: 10 mM; pH: 5.7; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available
13C-HSQCnot availablenot availablenot available
13C-HSQCTOCSYnot availablenot availablenot available

Software:

NMRPipe v2001 - processing

NMRView v5.0.4 - data analysis

DYANA v1.5 - structure refinement, structure solution

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

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