BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5913

Title: NMR SOLUTION STRUCTURE OF CN12, A NOVEL ALPHA-SCORPION TOXIN FROM CENTRUROIDES NOXIUS, SHOWING THE IMPORTANCE OF SURFACE CHARGE DISTRIBUTION FOR NA+-CHANNEL AFINITY.   PubMed: 15182366

Authors: DEL RIO-PORTILLA, F.; POSSANI, L.; HERNANDEZ-MARIN, E.; WANKE, E.; PIMIENTA, G.; CORONAS, F.; ZAMUDIO, F.

Citation: DEL RIO-PORTILLA, F.; HERNANDEZ-MARIN, E.; PIMIENTA, G.; CORONAS, F.; ZAMUDIO, F.; Rodriguez de la Vega, R.; Wanke, E.; Possani, L.. "NMR solution structure of Cn12, a novel peptide from the Mexican scorpion Centruroides noxius with a typical beta-toxin sequence but with alpha-like physiological activity"  Eur. J. Biochem. 271, 2504-2516 (2004).

Assembly members:
Neurotoxin Cn11, polymer, 67 residues, Formula weight is not available

Natural source:   Common Name: Mexican scorpion   Taxonomy ID: 6878   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Centruroides noxius

Experimental source:   Production method: .

Entity Sequences (FASTA):
Neurotoxin Cn11: RDGYPLASNGCKFGCSGLGE NNPTCNHVCEKKAGSDYGYC YAWTCYCEHVAEGTVLWGDS GTGPCRS

Data sets:
Data typeCount
coupling constants22

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Neurotoxin Cn111

Entities:

Entity 1, Neurotoxin Cn11 67 residues - Formula weight is not available

1   ARGASPGLYTYRPROLEUALASERASNGLY
2   CYSLYSPHEGLYCYSSERGLYLEUGLYGLU
3   ASNASNPROTHRCYSASNHISVALCYSGLU
4   LYSLYSALAGLYSERASPTYRGLYTYRCYS
5   TYRALATRPTHRCYSTYRCYSGLUHISVAL
6   ALAGLUGLYTHRVALLEUTRPGLYASPSER
7   GLYTHRGLYPROCYSARGSER

Samples:

sample_1: Neurotoxin Cn11 0.6 mM; H2O 90%; D2O 10%

sample_2: Neurotoxin Cn11 0.6 mM; D2O 100%

sample_cond_1: pH: 3.1; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
DQF COSYnot availablenot availablesample_cond_1
2D TOCSYnot availablenot availablesample_cond_1
AND 2D NOSEYnot availablenot availablesample_cond_1
DQF-COSYnot availablenot availablesample_cond_1
AND 2D NOESYnot availablenot availablesample_cond_1

Software:

CNS v1.1 - refinement

NMRPipe v1 - processing

VNMR v6.1c - collection

NMR spectrometers:

  • VARIAN UNITY PLUS 500 MHz

Related Database Links:

PDB
SP P63019