BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5917

Title: Backbone 1HN, 13C, 15N Chemical Shift Assignments for human eIF2alpha

Authors: Ito, Takuhiro

Citation: Ito, Takuhiro; Wagner, Gerhard. "Using condon optimization, chaperone co-expression, and rational mutagenesis for production and NMR assignments of Human eIF2a"  J. Biomol. NMR 28, 357-367 (2004).

Assembly members:
eIF2alpha, polymer, 308 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
eIF2alpha: MSCRFYQHKFPEVEDVVMVN VRSIQEMGAYVSLLEYNNIE GMIHLSELSRRRIRSINKLI RIGRNECVKVIRVDKEKGYI DLSKRRVSPEEAIKCEDKFT KSKTVYSILRHVAEVLEYTK DEQLESLFQRTAWVFDDKYK RPGYGAYDAFKHAVSDPSIL DSLDLNEDEREVLINNINRR LTPQAVKIRADIEVACYGYE GIDAVKEALRAGLNCSTENM PIKINLIAPPRYVMTTTTLE RTEGLSVLSQAMAVIKEKIE EKRGVFNVQMEPKVVTDTDE TELARQMERLERENAEVDGD LEHHHHHH

Data sets:
Data typeCount
1H chemical shifts278
13C chemical shifts566
15N chemical shifts278

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1heIF2alpha monomer1

Entities:

Entity 1, heIF2alpha monomer 308 residues - Formula weight is not available

1   METSERCYSARGPHETYRGLNHISLYSPHE
2   PROGLUVALGLUASPVALVALMETVALASN
3   VALARGSERILEGLNGLUMETGLYALATYR
4   VALSERLEULEUGLUTYRASNASNILEGLU
5   GLYMETILEHISLEUSERGLULEUSERARG
6   ARGARGILEARGSERILEASNLYSLEUILE
7   ARGILEGLYARGASNGLUCYSVALLYSVAL
8   ILEARGVALASPLYSGLULYSGLYTYRILE
9   ASPLEUSERLYSARGARGVALSERPROGLU
10   GLUALAILELYSCYSGLUASPLYSPHETHR
11   LYSSERLYSTHRVALTYRSERILELEUARG
12   HISVALALAGLUVALLEUGLUTYRTHRLYS
13   ASPGLUGLNLEUGLUSERLEUPHEGLNARG
14   THRALATRPVALPHEASPASPLYSTYRLYS
15   ARGPROGLYTYRGLYALATYRASPALAPHE
16   LYSHISALAVALSERASPPROSERILELEU
17   ASPSERLEUASPLEUASNGLUASPGLUARG
18   GLUVALLEUILEASNASNILEASNARGARG
19   LEUTHRPROGLNALAVALLYSILEARGALA
20   ASPILEGLUVALALACYSTYRGLYTYRGLU
21   GLYILEASPALAVALLYSGLUALALEUARG
22   ALAGLYLEUASNCYSSERTHRGLUASNMET
23   PROILELYSILEASNLEUILEALAPROPRO
24   ARGTYRVALMETTHRTHRTHRTHRLEUGLU
25   ARGTHRGLUGLYLEUSERVALLEUSERGLN
26   ALAMETALAVALILELYSGLULYSILEGLU
27   GLULYSARGGLYVALPHEASNVALGLNMET
28   GLUPROLYSVALVALTHRASPTHRASPGLU
29   THRGLULEUALAARGGLNMETGLUARGLEU
30   GLUARGGLUASNALAGLUVALASPGLYASP
31   LEUGLUHISHISHISHISHISHIS

Samples:

sample_1: eIF2alpha, [U-2H; U-13C; U-15N], 0.5 – 0.7 mM

condition_1: pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Varian Inova 750 MHz
  • Bruker Avance 600 MHz
  • Bruker Avance 500 MHz

Related Database Links:

BMRB 10023
PDB
DBJ BAB27049 BAE25400 BAE39796 BAE89774 BAG35351
EMBL CAA37728 CAD61953 CAH93423
GB AAA41110 AAA52373 AAH02513 AAH05463 AAH16448
REF NP_001127006 NP_001244395 NP_001270066 NP_004085 NP_062229
SP P05198 P68101 P68102 Q5R493 Q6ZWX6
TPG DAA25059