BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5922

Title: Solution Structure of the HERG K+ channel S5-P extracellular linker   PubMed: 12902341

Authors: Torres, A.; Bansal, P.; Sunde, M.; Clarke, C.; Bursill, J.; Smith, D.; Bauskin, A.; Breit, S.; Campbell, T.; Alewood, P.; Kuchel, P.; Vandenberg, J.

Citation: Torres, A.; Bansal, P.; Sunde, M.; Clarke, C.; Bursill, J.; Smith, D.; Bauskin, A.; Breit, S.; Campbell, T.; Alewood, P.; Kuchel, P.; Vandenberg, J.. "Structure of the HERG K+ channel S5P extracellular linker: Role of an amphipathic alpha-helix in c-type inactivation"  J. Biol. Chem. 278, 42136-42148 (2003).

Assembly members:
HERG S5-P linker, polymer, 42 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
HERG S5-P linker: AIGNXEQPHXDSRIGWLHNL GDQIGKPYNSSGLGGPSIKD KY

Data sets:
Data typeCount
1H chemical shifts250

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Potassium voltage-gated channel subfamily H member 21

Entities:

Entity 1, Potassium voltage-gated channel subfamily H member 2 42 residues - Formula weight is not available

1   ALAILEGLYASNNLEGLUGLNPROHISNLE
2   ASPSERARGILEGLYTRPLEUHISASNLEU
3   GLYASPGLNILEGLYLYSPROTYRASNSER
4   SERGLYLEUGLYGLYPROSERILELYSASP
5   LYSTYR

Samples:

sample_1: HERG S5-P linker 1.4 mM; SDS 100 mM; D2O 10%; H2O 90%

sample_cond_1: pH: 3.3; temperature: 303 K; ionic strength: 0 M; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availablesample_cond_1

Software:

XWINNMR v3.1 - processing

XEASY v1.3.13 - data analysis

DYANA v1.5 - structure solution

CNS v1.1 - refinement

NMR spectrometers:

  • Bruker AVANCE 600 MHz

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