BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5923

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for T22G mutant of N-terminal SH3 domain of the Drosophila protein drk   PubMed: 16300404

Authors: Bezsonova, Irina; Singer, Alex; Choy, Wing-Yiu; Tollinger, Martin; Forman-Kay, Julie

Citation: Bezsonova, Irina; Singer, Alex; Choy, Wing-Yiu; Tollinger, Martin; Forman-Kay, Julie. "Structural comparison of the unstable drkN SH3 domain and a stable mutant"  Biochemistry 44, 15550-15560 (2005).

Assembly members:
N-terminal SH3 domain of the Drosophila adapter protein drk, polymer, 59 residues, Formula weight is not available

Natural source:   Common Name: Fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
N-terminal SH3 domain of the Drosophila adapter protein drk: MEAIAKHDFSATADDELSFR KGGILKILNMGDDSNWYRAE LDGKEGLIPSNYIEMKNHD

Data sets:
Data typeCount
13C chemical shifts51
15N chemical shifts51
1H chemical shifts51

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1T22G mutant of N-terminal SH3 domain of SH2-SH3 adapter protein drk1

Entities:

Entity 1, T22G mutant of N-terminal SH3 domain of SH2-SH3 adapter protein drk 59 residues - Formula weight is not available

1   METGLUALAILEALALYSHISASPPHESER
2   ALATHRALAASPASPGLULEUSERPHEARG
3   LYSGLYGLYILELEULYSILELEUASNMET
4   GLYASPASPSERASNTRPTYRARGALAGLU
5   LEUASPGLYLYSGLUGLYLEUILEPROSER
6   ASNTYRILEGLUMETLYSASNHISASP

Samples:

sample_1: N-terminal SH3 domain of the Drosophila adapter protein drk, [U-13C; U-15N], 1.0 mM

Ex-cond_1: pH: 6.0; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCOnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian INOVA 500 MHz

Related Database Links:

BMRB 5925