BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5925

Title: Solution structure of the N-terminal SH3 domain of Drk (drkN SH3 domain)   PubMed: 16300404

Authors: Bezsonova, I.; Singer, A.; Choy, W.-Y.; Tollinger, M.; Forman-Kay, J.

Citation: Bezsonova, Irina; Singer, Alex; Choy, Wing-Yiu; Tollinger, Martin; Forman-Kay, Julie. "Structural comparison of the unstable drkN SH3 domain and a stable mutant"  Biochemistry 44, 15550-15560 (2005).

Assembly members:
The Drosophila N-terminal SH3 domain of Downstream of Receptor Kinases protein, polymer, 59 residues, Formula weight is not available

Natural source:   Common Name: Fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
The Drosophila N-terminal SH3 domain of Downstream of Receptor Kinases protein: MEAIAKHDFSATADDELSFR KTGILKILNMGDDSNWYRAE LDGKEGLIPSNYIEMKNHD

Data sets:
Data typeCount
13C chemical shifts56
15N chemical shifts56
1H chemical shifts56

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1SH2-SH3 adapter protein drk1

Entities:

Entity 1, SH2-SH3 adapter protein drk 59 residues - Formula weight is not available

1   METGLUALAILEALALYSHISASPPHESER
2   ALATHRALAASPASPGLULEUSERPHEARG
3   LYSTHRGLYILELEULYSILELEUASNMET
4   GLYASPASPSERASNTRPTYRARGALAGLU
5   LEUASPGLYLYSGLUGLYLEUILEPROSER
6   ASNTYRILEGLUMETLYSASNHISASP

Samples:

sample_1: The Drosophila N-terminal SH3 domain of Downstream of Receptor Kinases protein, [U-13C; U-15N], 1.0 mM; Na2SO4 500 mM; phosphate buffer 50 mM; D2O 10%; H2O 90%

sample_cond_1: ionic strength: 1.664 M; pH: 6.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions

Software:

NMRPipe - processing

CNS v1.0 - refinement

PIPP - data analysis

NMR spectrometers:

  • Varian INOVA 500 MHz

Related Database Links:

BMRB 5923