BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5929

Title: NMR assignment of the hypothetical rhodanase domain At4g01050 from Arabidopsis thaliana   PubMed: 15014235

Authors: Pantoja-Uceda, David; Lopez-Mendez, Blanca; Koshiba, Seizo; Kigawa, Takanori; Shirouzu, Mikako; Terada, Takaho; Inoue, Makoto; Yabuki, Takashi; Aoki, Masaaki; Seki, Eiko; Matsuda, Takayoshi; Hirota, Hiroshi; Yoshida, Mayumi; Tanaka, Akiko; Osanai, Takashi; Seki, Motoaki; Shinozaki, Kazuo; Yokoyama, Shigeyuki; Guntert, Peter

Citation: Pantoja-Uceda, David; Lopez-Mendez, Blanca; Koshiba, Seizo; Kigawa, Takanori; Shirouzu, Mikako; Terada, Takaho; Inoue, Makoto; Yabuki, Takashi; Aoki, Masaaki; Seki, Eiko; Matsuda, Takayoshi; Hirota, Hiroshi; Yoshida, Mayumi; Tanaka, Akiko; Osanai, Takashi; Seki, Motoaki; Shinozaki, Kazuo; Yokoyama, Shigeyuki; Guntert, Peter. "Letter to the Editor: NMR assignment of the hypothetical rhodanese domain At4g01050 from Arabidopsis thaliana "  J. Biomol. NMR 29, 207-208 (2004).

Assembly members:
single chain biopolymer, polymer, 134 residues, Formula weight is not available

Natural source:   Common Name: Thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
single chain biopolymer: GSSGSSGSAKNAYTKLGTDD NAQLLDIRATADFRQVGSPN IKGLGKKAVSTVYNGEDKPG FLKKLSLKFKDPENTTLYIL DKFDGNSELVAELVALNGFK SAYAIKDGAEGPRGWLNSSL PWIEPKKTSGPSSG

Data sets:
Data typeCount
1H chemical shifts908
13C chemical shifts559
15N chemical shifts142

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1rhodanase monomer1

Entities:

Entity 1, rhodanase monomer 134 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYSERALALYS
2   ASNALATYRTHRLYSLEUGLYTHRASPASP
3   ASNALAGLNLEULEUASPILEARGALATHR
4   ALAASPPHEARGGLNVALGLYSERPROASN
5   ILELYSGLYLEUGLYLYSLYSALAVALSER
6   THRVALTYRASNGLYGLUASPLYSPROGLY
7   PHELEULYSLYSLEUSERLEULYSPHELYS
8   ASPPROGLUASNTHRTHRLEUTYRILELEU
9   ASPLYSPHEASPGLYASNSERGLULEUVAL
10   ALAGLULEUVALALALEUASNGLYPHELYS
11   SERALATYRALAILELYSASPGLYALAGLU
12   GLYPROARGGLYTRPLEUASNSERSERLEU
13   PROTRPILEGLUPROLYSLYSTHRSERGLY
14   PROSERSERGLY

Samples:

sample_1: single chain biopolymer, [U-13C; U-15N], 1.10 mM; phosphate buffer 20 mM; NaCl 100 mM; dithiothreitol 1 mM; sodium azide 0.02 % (v/v); H2O 90 % (v/v); D2O 10 % (v/v)

condition_set_1: pH: 6.0 na; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
[1H,15N ]-HSQCnot availablenot availablenot available
[1H,13C]-HSQCnot availablenot availablenot available
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
HNCAnot availablenot availablenot available
HA-coupled HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNHAnot availablenot availablenot available
HBHA(CO)NHnot availablenot availablenot available
(H)CC(CO)NHnot availablenot availablenot available
HCCH-COSYnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
H(CCCO)NHnot availablenot availablenot available
3D 15N-edited [1H,1H]-NOESYnot availablenot availablenot available
13C-edited [1H,1H]-NOESYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker AV 800 MHz

Related Database Links:

PDB
DBJ BAE98457
EMBL CAB80914
GB AAB61039 AAL09804 AAL15331 AAM70579 AEE81973
REF NP_567209 XP_010424692
SP Q9M158