BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5932

Title: YNMG tetraloop formation by a dyskeratosis congenita mutation in human telomerase RNA   PubMed: 14624001

Authors: Theimer, C.; Finger, L.; Feigon, J.

Citation: Theimer, C.; Finger, L.; Feigon, J.. "YNMG tetraloop formation by a dyskeratosis congenita mutation in human telomerase RNA"  RNA 9, 1446-1455 (2003).

Assembly members:
human telomerase RNA P2b loop, polymer, 15 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):
human telomerase RNA P2b loop: GGCUCUCAGUGAGCC

Data sets:
Data typeCount
13C chemical shifts100
15N chemical shifts31
1H chemical shifts136

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1dcloop1

Entities:

Entity 1, dcloop 15 residues - Formula weight is not available

1   GGCUCUCAGU
2   GAGCC

Samples:

sample_1: human telomerase RNA P2b loop 1 mM; sodium phosphate buffer 10 mM; KCl 200 mM; EDTA 50 uM; sodium azide 0.2%; H2O 95%; D2O 5%

sample_2: human telomerase RNA P2b loop, [U-13C; U-15N], 1 mM; sodium phosphate buffer 10 mM; KCl 200 mM; EDTA 50 uM; sodium azide 0.2%; H2O 95%; D2O 5%

sample_3: human telomerase RNA P2b loop 1 mM; sodium phosphate buffer 10 mM; KCl 200 mM; EDTA 50 uM; sodium azide 0.2%; D2O 100%

sample_4: human telomerase RNA P2b loop, [U-13C; U-15N], 1 mM; sodium phosphate buffer 10 mM; KCl 200 mM; EDTA 50 uM; sodium azide 0.2%; D2O 100%

sample_cond_1: ionic strength: 200 mM; pH: 6.3; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D NOESY (1,1echo and watergate)not availablenot availablenot available
2D 15N-HMQCnot availablenot availablenot available
2D 15N-CPMG-NOESYnot availablenot availablenot available
2D JNN-HNN-COSYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
31P-spin echo difference CT HSQCnot availablenot availablenot available
3D 13C-NOESY HMQCnot availablenot availablenot available
2D 13C-HMQCnot availablenot availablenot available
2D HCCH COSYnot availablenot availablenot available
3D HCCH TOCSYnot availablenot availablenot available
CT-CE-HSQCnot availablenot availablenot available

Software:

xwinnmr v2.6 - collection, processing

AURELIA v3.108 - data analysis

X-PLOR v3.851 - refinement, structure solution

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 500 MHz

Related Database Links:

BMRB 4093 5347 5931
PDB
DBJ BAA12997 BAH96515 BAH96524
GB AAA93161 AAA93162 AAA93165 AAB05599 AAB50259
PIR GNVWLV
REF NP_057849 NP_705927 NP_789740 YP_001856242
SP P03367 P04585