BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5935

Title: Sequence-specific 1H, 13C and 15N resonance assignments of rat liver fructose-2,6-bisphosphatase domain

Authors: Zangger, Klaus; Pervushin, Konstantin; Sterk, Heinz; Lange, Alex; Okar, David

Citation: Zangger, Klaus; Pervushin, Konstantin; Sterk, Heinz; Lange, Alex; Okar, David. "Sequence-specific 1H, 13C and 15N resonance assignments of rat liver fructose-2,6-bisphosphatase domain "  J. Biomol. NMR 27, 281-282 (2003).

Assembly members:
Fructose-2,6-bisphosphatase, polymer, 221 residues, 25554 Da.

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Fructose-2,6-bisphosphatase: MRSIYLCRHGESELNLRGRI GGDSGLSARGKQYAYALANF IRSQGISSLKVWTSHMKRTI QTAEALGVPYEQWKALNEID AGVCEEMTYEEIQEHYPEEF ALRDQDKYRYRYPKGESYED LVQRLEPVIMELERQENVLV ICHQAVMRCLLAYFLDKSSD ELPYLKCPLHTVLKLTPVAY GCRVESIYLNVEAVNTHRDK PENVDITREAEEALDTVPAH Y

Data sets:
Data typeCount
1H chemical shifts163
13C chemical shifts558
15N chemical shifts163

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FBP-2 monomer1

Entities:

Entity 1, FBP-2 monomer 221 residues - 25554 Da.

1   METARGSERILETYRLEUCYSARGHISGLY
2   GLUSERGLULEUASNLEUARGGLYARGILE
3   GLYGLYASPSERGLYLEUSERALAARGGLY
4   LYSGLNTYRALATYRALALEUALAASNPHE
5   ILEARGSERGLNGLYILESERSERLEULYS
6   VALTRPTHRSERHISMETLYSARGTHRILE
7   GLNTHRALAGLUALALEUGLYVALPROTYR
8   GLUGLNTRPLYSALALEUASNGLUILEASP
9   ALAGLYVALCYSGLUGLUMETTHRTYRGLU
10   GLUILEGLNGLUHISTYRPROGLUGLUPHE
11   ALALEUARGASPGLNASPLYSTYRARGTYR
12   ARGTYRPROLYSGLYGLUSERTYRGLUASP
13   LEUVALGLNARGLEUGLUPROVALILEMET
14   GLULEUGLUARGGLNGLUASNVALLEUVAL
15   ILECYSHISGLNALAVALMETARGCYSLEU
16   LEUALATYRPHELEUASPLYSSERSERASP
17   GLULEUPROTYRLEULYSCYSPROLEUHIS
18   THRVALLEULYSLEUTHRPROVALALATYR
19   GLYCYSARGVALGLUSERILETYRLEUASN
20   VALGLUALAVALASNTHRHISARGASPLYS
21   PROGLUASNVALASPILETHRARGGLUALA
22   GLUGLUALALEUASPTHRVALPROALAHIS
23   TYR

Samples:

sample_1: Fructose-2,6-bisphosphatase, [U-95% 13C; U-95 15N], 2.7 mM; sodium phosphate 100 mM

sample_2: Fructose-2,6-bisphosphatase 1.5 mM; sodium phosphate 100 mM

Ex-cond_1: pH: 7.0; temperature: 298 K; ionic strength: 1.2 M

Experiments:

NameSampleSample stateSample conditions
TROSY versions used for all experiments;not availablenot availableEx-cond_1
2H decoupling used on triply-labeled sample.not availablenot availableEx-cond_1

Software:

NMRPipe v03/2002 - processing

NMRView v5.0.3 - analysis

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Varian INOVA 600 MHz
  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAE21271 BAG36785 BAG62397
EMBL CAA33606 CAA33607 CAA68694
GB AAA30696 AAA40624 AAA79008 AAB19845 AAH96077
PIR S77704
REF NP_001257992 NP_001257993 NP_001258733 NP_001258734 NP_002616
SP P07953 P16118 P49872 P70266
TPG DAA12796