BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5944

Title: A Zinc Clasp Structure Tethers Lck to T Cell Coreceptors CD4 and CD8   PubMed: 14500983

Authors: Kim, Peter; Sun, Zhen-Yu; Blacklow, Stephen; Wagner, Gerhard; Eck, Michael

Citation: Kim, Peter; Sun, Zhen-Yu; Blacklow, Stephen; Wagner, Gerhard; Eck, Michael. "A Zinc Clasp Structure Tethers Lck to T Cell Coreceptors CD4 and CD8"  Science (Washington, DC, U. S.) 301, 1725-1728 (2003).

Assembly members:
CD4, polymer, 38 residues, Formula weight is not available
Lck, polymer, 29 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
CD4: RCRHRRRQAERLSQIKRLLS EKKTCQCPHRFQKTCSPI
Lck: SHPEDDWLENIDVCENCHYP IVPLDGKGT

Data sets:
Data typeCount
13C chemical shifts195
15N chemical shifts55
1H chemical shifts359

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CD4 cytoplasmic tail1
2Lck N-terminus2
3ZINC (II) ION3

Entities:

Entity 1, CD4 cytoplasmic tail 38 residues - Formula weight is not available

1   ARGCYSARGHISARGARGARGGLNALAGLU
2   ARGLEUSERGLNILELYSARGLEULEUSER
3   GLULYSLYSTHRCYSGLNCYSPROHISARG
4   PHEGLNLYSTHRCYSSERPROILE

Entity 2, Lck N-terminus 29 residues - Formula weight is not available

1   SERHISPROGLUASPASPTRPLEUGLUASN
2   ILEASPVALCYSGLUASNCYSHISTYRPRO
3   ILEVALPROLEUASPGLYLYSGLYTHR

Entity 3, ZINC (II) ION - Zn - 65.409 Da.

1   ZN

Samples:

Sample_1: CD4 cytoplasmic tail - Lck N terminus 0.5 mM; Lck 0.5 mM

Sample_2: CD4 cytoplasmic tail - Lck N terminus, [U-15N], 0.5 mM; Lck 0.5 mM

Sample_3: CD4 cytoplasmic tail - Lck N terminus, [U-15N; U-13C], 0.5 mM; Lck 0.5 mM

Condition_set_1: pH: 5.1; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availableCondition_set_1
HN(CO)CAnot availablenot availableCondition_set_1
HNCACBnot availablenot availableCondition_set_1
HN(CO)CACBnot availablenot availableCondition_set_1
CCONHnot availablenot availableCondition_set_1
HCCONHnot availablenot availableCondition_set_1
HCCH TOCSYnot availablenot availableCondition_set_1
15N-NOESYnot availablenot availableCondition_set_1
15N-TOCSYnot availablenot availableCondition_set_1
D2O NOESYnot availablenot availableCondition_set_1
D2O TOCSYnot availablenot availableCondition_set_1
Cd-H HMQCnot availablenot availableCondition_set_1
Cd-H NOESY-HMQCnot availablenot availableCondition_set_1

Software:

FELIX - processing

DYANA - structure solution

X-PLOR - structure solution

NMR spectrometers:

  • Bruker Varian 500 MHz
  • Bruker Varian 750 MHz

Related Database Links:

PDB
BMRB 5945