BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5945

Title: A Zinc Clasp Structure Tethers Lck to T Cell Coreceptors CD4 and CD8   PubMed: 14500983

Authors: Kim, Peter; Sun, Zhen-Yu; Blacklow, Stephen; Wagner, Gerhard; Eck, Michael

Citation: Kim, Peter; Sun, Zhen-Yu; Blacklow, Stephen; Wagner, Gerhard; Eck, Michael. "A Zinc Clasp Structure Tethers Lck to T Cell Coreceptors CD4 and CD8"  Science (Washington, DC, U. S.) 301, 1725-1728 (2003).

Assembly members:
CD8a, polymer, 19 residues, Formula weight is not available
Lck, polymer, 29 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
CD8a: RNRRRVCKCPRPVVKSGDK
Lck: SHPEDDWLENIDVCENCHYP IVPLDGKGT

Data sets:
Data typeCount
1H chemical shifts268
13C chemical shifts148
15N chemical shifts41

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CD8a cytoplasmic tail1
2Lck N-terminus2
3ZINC (II) ION3

Entities:

Entity 1, CD8a cytoplasmic tail 19 residues - Formula weight is not available

1   ARGASNARGARGARGVALCYSLYSCYSPRO
2   ARGPROVALVALLYSSERGLYASPLYS

Entity 2, Lck N-terminus 29 residues - Formula weight is not available

1   SERHISPROGLUASPASPTRPLEUGLUASN
2   ILEASPVALCYSGLUASNCYSHISTYRPRO
3   ILEVALPROLEUASPGLYLYSGLYTHR

Entity 3, ZINC (II) ION - Zn - 65.409 Da.

1   ZN

Samples:

Sample_1: CD8a 0.5 mM; Lck 0.5 mM

Condition_set_1: pH: 5.1; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCASample_1not availableCondition_set_1
HN(CO)CASample_1not availableCondition_set_1
HNCACBSample_1not availableCondition_set_1
HN(CO)CACBSample_1not availableCondition_set_1
CCONHSample_1not availableCondition_set_1
HCCONHSample_1not availableCondition_set_1
HCCH TOCSYSample_1not availableCondition_set_1
15N-NOESYSample_1not availableCondition_set_1
15N-TOCSYSample_1not availableCondition_set_1
D2O NOESYSample_1not availableCondition_set_1
D2O TOCSYSample_1not availableCondition_set_1
Cd-H HMQCSample_1not availableCondition_set_1
Cd-H NOESY-HMQCSample_1not availableCondition_set_1

Software:

FELIX - processing

DYANA - structure solution

X-PLOR - structure solution

NMR spectrometers:

  • Bruker Varian 500 MHz
  • Bruker Varian 750 MHz

Related Database Links:

SWISS-PROT P01732
REF XP_001138871 XP_001138772 NP_741969 NP_001759
GenBank AAB04637 AAA79218 AAA79217 AAA61133 AAA59674
DBJ BAG61892 BAG53555
PDB
BMRB 5944