BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5947

Title: Complete resonance assignments of a 'donor strand complemented' AfaE-III: the afimbrial adhesin from Diffusely Adherent E. coli   PubMed: 15213443

Authors: Anderson, Kirstine; Cota, Ernesto; Simpson, Peter; Chen, Ho An; Le Bouguenec, Chantal; Matthews, Stephen

Citation: Anderson, Kirstine; Cota, Ernesto; Simpson, Peter; Chen, Ho An; du Merle, Laurence; Le Bouguenec, Chantal; Matthews, Stephen. "Letter to the Editor: Complete resonance assignments of a 'donor strand complemented' AfaE: The afimbrial adhesin from Diffusely Adherent E. coli"  J. Biomol. NMR 29, 409-410 (2004).

Assembly members:
donor strand complemented AfaE-III, polymer, 156 residues, 16932.7 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
donor strand complemented AfaE-III: HHHHHHGLVPRGSEECQVRV GDLTVAKTRGQLTDAAPIGP VTVQALGCNARQVALKADTD NFEQGKFFLISDNNRDKLYV NIRPMDNSAWTTDNGVFYKN DVGSWGGTIGIYVDGQQTNT PPGNYTLTLTGGYWAKDNKQ GFTPSGTTGTTKLTVT

Data sets:
Data typeCount
13C chemical shifts576
15N chemical shifts146
1H chemical shifts843

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AfaE-dsc1

Entities:

Entity 1, AfaE-dsc 156 residues - 16932.7 Da.

1   HISHISHISHISHISHISGLYLEUVALPRO
2   ARGGLYSERGLUGLUCYSGLNVALARGVAL
3   GLYASPLEUTHRVALALALYSTHRARGGLY
4   GLNLEUTHRASPALAALAPROILEGLYPRO
5   VALTHRVALGLNALALEUGLYCYSASNALA
6   ARGGLNVALALALEULYSALAASPTHRASP
7   ASNPHEGLUGLNGLYLYSPHEPHELEUILE
8   SERASPASNASNARGASPLYSLEUTYRVAL
9   ASNILEARGPROMETASPASNSERALATRP
10   THRTHRASPASNGLYVALPHETYRLYSASN
11   ASPVALGLYSERTRPGLYGLYTHRILEGLY
12   ILETYRVALASPGLYGLNGLNTHRASNTHR
13   PROPROGLYASNTYRTHRLEUTHRLEUTHR
14   GLYGLYTYRTRPALALYSASPASNLYSGLN
15   GLYPHETHRPROSERGLYTHRTHRGLYTHR
16   THRLYSLEUTHRVALTHR

Samples:

sample_1: donor strand complemented AfaE-III, [U-95% 13C; U-95% 15N], 0.8 mM; acetate buffer 50 mM; sodium chloride 100 mM

conditions_1: pH: 5; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
HBHAnot availablenot availablenot available
(H)CC(CO)NH TOCSYnot availablenot availablenot available
H(CCCO)NH TOCSYnot availablenot availablenot available
HCCH TOCSYnot availablenot availablenot available
1H-13C NOESYnot availablenot availablenot available
1H-15N/13C simultanious NOESYnot availablenot availablenot available

Software:

ARIA v1.2 - automated NOE assignments, structure calulation

NMR spectrometers:

  • Bruker DMX 500 MHz
  • Varian Inova 800 MHz

Related Database Links:

BMRB 16417
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