BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5948

Title: Structure Analysis of Integrin alpha IIb beta 3 - Specific Disintegrin with the AKGDWN Motif

Authors: Chuang, Woei-Jer; Chen, Chiu-Yueh; Shiu, Jia-Hau; Liu, Yu-Chen

Citation: Chuang, Woei-Jer; Chen, Chiu-Yueh; Shiu, Jia-Hau; Liu, Yu-Chen. "Structure Analysis of Integrin alpha IIb beta 3 - Specific Disintegrin with the AKGDWN Motif"  . ., .-..

Assembly members:
Rhodostomin, polymer, 68 residues, Formula weight is not available

Natural source:   Common Name: Calloselasma rhodostoma   Taxonomy ID: 8717   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Calloselasma rhodostoma

Experimental source:   Production method: recombinant technology   Host organism: Pichia pastoris

Entity Sequences (FASTA):
Rhodostomin: GKECDCSSPENPCCDAATCK LRPGAQCGEGLCCEQCKFSR AGKICRIAKGDWNDDRCTGQ SADCPRYH

Data sets:
Data typeCount
15N chemical shifts64
1H chemical shifts375

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Disintegrin kistrin1

Entities:

Entity 1, Disintegrin kistrin 68 residues - Formula weight is not available

1   GLYLYSGLUCYSASPCYSSERSERPROGLU
2   ASNPROCYSCYSASPALAALATHRCYSLYS
3   LEUARGPROGLYALAGLNCYSGLYGLUGLY
4   LEUCYSCYSGLUGLNCYSLYSPHESERARG
5   ALAGLYLYSILECYSARGILEALALYSGLY
6   ASPTRPASNASPASPARGCYSTHRGLYGLN
7   SERALAASPCYSPROARGTYRHIS

Samples:

sample_1: Rhodostomin 3 mM; H2O 90%; D2O 10%

sample_2: Rhodostomin 3 mM; D2O 100%

sample_3: Rhodostomin, [U-15N], 2.5 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 0 M; pH: 6.0; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
3D 15N-separated TOCSYnot availablenot availablenot available
HNHAnot availablenot availablenot available
15N-HSQCnot availablenot availablenot available

Software:

xwinnmr v2.6 - processing

AURELIA v2.7.10 - data analysis

X-PLOR v3.85 - refinement

NMR spectrometers:

  • Bruker AVANCE 600 MHz

Related Database Links:

BMRB 19210 19211 19212 5949
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