BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5958

Title: 1H, 13C and 15N resonance assignments of human microtubule-associated protein light chain-3   PubMed: 15213446

Authors: Kouno, Takahide; Mizuguchi, Mineyuki; Tanida, Isei; Ueno, Takashi; Kominami, Eiki; Kawano, Keiichi

Citation: Kouno, Takahide; Mizuguchi, Mineyuki; Tanida, Isei; Ueno, Takashi; Kominami, Eiki; Kawano, Keiichi. "Letter to the Editor: 1H, 13C and 15N resonance assignments of human microtubule-associated protein light chain-3 "  J. Biomol. NMR 29, 415-416 (2004).

Assembly members:
microtubule-associated protein light chain-3, polymer, 120 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
microtubule-associated protein light chain-3: MPSEKTFKQRRTFEQRVEDV RLIREQHPTKIPVIIERYKG EKQLPVLDKTKFLVPDHVNM SELIKIIRRRLQLNANQAFF LLVNGHSMVSVSTPISEVYE SEKDEDGFLYMVYASQETFG

Data sets:
Data typeCount
1H chemical shifts237
13C chemical shifts235
15N chemical shifts114

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1MAP-LC3 monomer1

Entities:

Entity 1, MAP-LC3 monomer 120 residues - Formula weight is not available

1   METPROSERGLULYSTHRPHELYSGLNARG
2   ARGTHRPHEGLUGLNARGVALGLUASPVAL
3   ARGLEUILEARGGLUGLNHISPROTHRLYS
4   ILEPROVALILEILEGLUARGTYRLYSGLY
5   GLULYSGLNLEUPROVALLEUASPLYSTHR
6   LYSPHELEUVALPROASPHISVALASNMET
7   SERGLULEUILELYSILEILEARGARGARG
8   LEUGLNLEUASNALAASNGLNALAPHEPHE
9   LEULEUVALASNGLYHISSERMETVALSER
10   VALSERTHRPROILESERGLUVALTYRGLU
11   SERGLULYSASPGLUASPGLYPHELEUTYR
12   METVALTYRALASERGLNGLUTHRPHEGLY

Samples:

sample_1: microtubule-associated protein light chain-3, [U-13C; U-15N], 0.8 – 1.0 mM

sample_2: microtubule-associated protein light chain-30.8 – 1.0 mM

Ex-cond_1: pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H,15N-HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
CBCANHnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
H(COO)NHnot availablenot availablenot available
HBHA(CBCA)NHnot availablenot availablenot available
HBHA(CBCACO)NHnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker DMX 500 MHz

Related Database Links:

BMRB 15877 18518
PDB
DBJ BAB15169 BAB22364 BAB22569 BAB22641 BAB22855
EMBL CAD38970 CAG31435 CAL38438
GB AAA20645 AAB72082 AAG09686 AAG23182 AAH18634
REF NP_001001169 NP_001026632 NP_001078950 NP_001177219 NP_001180554
SP A6NCE7 O41515 Q62625 Q9CQV6 Q9GZQ8
TPG DAA20021