BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5960

Title: 1H, 13C, 15N- Chemical Shift Assignments for Myristoylated HIV-1 Matrix Protein   PubMed: 14699046

Authors: Tang, Chun; Loeliger, Erin; Luncsford, Paz; Kinde, Isaac; Beckett, Dorothy; Summers, Michael

Citation: Tang, Chun; Loeliger, Erin; Luncsford, Paz; Kinde, Isaac; Beckett, Dorothy; Summers, Michael. "From the Cover: Entropic switch regulates myristate exposure in the HIV-1 matrix protein"  Proc. Natl. Acad. Sci. U.S.A. 101, 517-522 (2004).

Assembly members:
p17, polymer, 132 residues, 228.371 Da.

Natural source:   Common Name: HIV-1   Taxonomy ID: 11676   Superkingdom: Viruses   Kingdom: not available   Genus/species: Lentivirus Human immunodeficiency virus 1

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
p17: XGARASVLSGGELDKWEKIR LRPGGKKQYKLKHIVWASRE LERFAVNPGLLETSEGCRQI LGQLQPSLQTGSEELRSLYN TIAVLYCVHQRIDVKDTKEA LDKIEEEQNKSKKKAQQAAA DTGNNSQVSQNY

Data sets:
Data typeCount
1H chemical shifts581
15N chemical shifts130
13C chemical shifts500

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1matrix monomer1

Entities:

Entity 1, matrix monomer 132 residues - 228.371 Da.

1   MYRGLYALAARGALASERVALLEUSERGLY
2   GLYGLULEUASPLYSTRPGLULYSILEARG
3   LEUARGPROGLYGLYLYSLYSGLNTYRLYS
4   LEULYSHISILEVALTRPALASERARGGLU
5   LEUGLUARGPHEALAVALASNPROGLYLEU
6   LEUGLUTHRSERGLUGLYCYSARGGLNILE
7   LEUGLYGLNLEUGLNPROSERLEUGLNTHR
8   GLYSERGLUGLULEUARGSERLEUTYRASN
9   THRILEALAVALLEUTYRCYSVALHISGLN
10   ARGILEASPVALLYSASPTHRLYSGLUALA
11   LEUASPLYSILEGLUGLUGLUGLNASNLYS
12   SERLYSLYSLYSALAGLNGLNALAALAALA
13   ASPTHRGLYASNASNSERGLNVALSERGLN
14   ASNTYR

Samples:

sample_1: p17, [U-13C; U-15N], 0.2 mM; sodium phosophate buffer 50 mM; NaCl 50 mM; DTT 5 mM; DTT 5 mM

cond_1: pH: 5.5; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1not availablecond_1
2D 1H-13C HMQCsample_1not availablecond_1
2D 1H-1H NOESYsample_1not availablecond_1
3D 13C-edited HMQC-NOESYsample_1not availablecond_1
2D 13C-edited HMQC-NOESYsample_1not availablecond_1
4D 13C, 13C-edited HMQC-NOESY-HMQCsample_1not availablecond_1
3D 15N- edited NOESY-HSQCsample_1not availablecond_1
3D HNCAsample_1not availablecond_1
3D HN(CO)CAsample_1not availablecond_1
3D HNCOsample_1not availablecond_1

Software:

NMRPIPE - processing

NMRVIEW v5.0.4 - processing

NMR spectrometers:

  • Bruker AVANCE 800 MHz
  • Bruker AVANCE 600 MHz

Related Database Links:

BMRB 15114 15116 18715 18716 5316 7250 7267 7275 7309
PDB
DBJ BAF34641 BAG48474
EMBL CBI61180 CBI61181 CBI61182 CBI61183 CBI61184
GB AAA44987 AAB00898 AAB60571 AAC28445 AAC29216
SP P12493 P12497