BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5961

Title: Binding site structure of one LRP/RAP complex - implications for a common ligand/receptor binding motif   PubMed: 16938309

Authors: Jensen, Gitte; Andersen, Olav; Bonvin, Alexandre; Bjerrum-Bohr, Ida; Etzeroth, Michael; Thoegersen, Hans; Poulsen, Flemming; Kragelund, Birthe

Citation: Jensen, Gitte; Andersen, Olav; Bonvin, Alexandre; Bjerrum-Bohr, Ida; Etzerodt, Michael; Thoegersen, Hans; O'Shea, C.; Poulsen, Flemming; Kragelund, Birthe. "Binding site structure of one LRP-RAP complex: implications for a common ligand-receptor binding motif"  J. Mol. Biol. 362, 700-716 (2006).

Assembly members:
complement type repeats 5 and 6, polymer, 82 residues, 8938.61 Da.
CA, non-polymer, 40.078 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichis coli

Entity Sequences (FASTA):
complement type repeats 5 and 6: SARTCPPNQFSCASGRCIPI SWTCDLDDDCGDRSDESASC AYPTCFPLTQFTCNNGRCIN INWRCDNDNDCGDNSDEAGC SH

Data sets:
Data typeCount
15N chemical shifts80
1H chemical shifts436

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CR561
2CALCIUM (II) ION, 12
3CALCIUM (II) ION, 22

Entities:

Entity 1, CR56 82 residues - 8938.61 Da.

1   SERALAARGTHRCYSPROPROASNGLNPHE
2   SERCYSALASERGLYARGCYSILEPROILE
3   SERTRPTHRCYSASPLEUASPASPASPCYS
4   GLYASPARGSERASPGLUSERALASERCYS
5   ALATYRPROTHRCYSPHEPROLEUTHRGLN
6   PHETHRCYSASNASNGLYARGCYSILEASN
7   ILEASNTRPARGCYSASPASNASPASNASP
8   CYSGLYASPASNSERASPGLUALAGLYCYS
9   SERHIS

Entity 2, CALCIUM (II) ION, 1 - Ca - 40.078 Da.

1   CA

Samples:

sample_1: complement type repeats 5 and 6 0.5 mM; CaCl2 10 mM; MgCl2 1 mM; NaCl 100 mM

sample_2: complement type repeats 5 and 6, [U-99% 15N], 0.5 mM; CaCl2 10 mM; MgCl2 1 mM; NaCl 100 mM

conditions: pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
DQF-COSYnot availablenot availableconditions
NOESYnot availablenot availableconditions
TOCSYnot availablenot availableconditions
15N-HSQCnot availablenot availableconditions
15N-NOESY-HSQCnot availablenot availableconditions
15N-TOCSY-HSQCnot availablenot availableconditions

Software:

PRONTO v20020517 - assignment tool

NMR spectrometers:

  • Varian UNITY 750 MHz
  • Varian UNITY 800 MHz

Related Database Links:

BMRB 17055
PDB