BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5968

Title: NMR structure of the human dematin headpiece domain   PubMed: 14660664

Authors: Frank, B.; Vardar, D.; Chishti, A.; McKnight, C.

Citation: Frank, B.; Vardar, D.; Chishti, A.; McKnight, C.. "The NMR Structure Dematin Headpiece Reveals a Dynamic Loop that is Conformationally Altered Upon Phosphorylation at a Distal Site "  J. Biol. Chem. ., .-..

Assembly members:
dematin, polymer, 68 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
dematin: PGLQIYPYEMLVVTNKGRTK LPPGVDRMRLERHLSAEDFS RVFAMSPEEFGKLALWKRNE LKKKASLF

Data sets:
Data typeCount
1H chemical shifts487
13C chemical shifts291
15N chemical shifts73

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1dematin1

Entities:

Entity 1, dematin 68 residues - Formula weight is not available

1   PROGLYLEUGLNILETYRPROTYRGLUMET
2   LEUVALVALTHRASNLYSGLYARGTHRLYS
3   LEUPROPROGLYVALASPARGMETARGLEU
4   GLUARGHISLEUSERALAGLUASPPHESER
5   ARGVALPHEALAMETSERPROGLUGLUPHE
6   GLYLYSLEUALALEUTRPLYSARGASNGLU
7   LEULYSLYSLYSALASERLEUPHE

Samples:

sample_1: dematin, [U-13C; U-15N], 1 mM; phosphat 10 mM; TMSP 0.5 mM; sodium azide 0.01%; D2O 10%

sample_2: dematin, [U-15N], 4 mM; phosphat 10 mM; TMSP 0.5 mM; sodium azide 0.01%; D2O 10%

sample_3: dematin, [U-10% 13C; U-15N], 0.7 mM; phosphat 10 mM; TMSP 0.5 mM; sodium azide 0.01%; D2O 10%

sample_cond_1: pH: 6.0; temperature: 293 K; ionic strength: 10 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCOCAnot availablenot availablenot available
HNCOnot availablenot availablenot available
HNCACOnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
3D 15N-separated TOCSYnot availablenot availablenot available
HCCH-COSYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available

Software:

CNS v1.0 - structure solution

NMRPipe vsgi6x - processing

XWINNMR v3.1 - collection

NMRView v4.1.2 - data analysis

MOLMOL v2k.2 - refinement

NMR spectrometers:

  • Bruker DMX 500 MHz

Related Database Links:

BMRB 6718
PDB
DBJ BAD92955 BAE28799 BAE34638 BAE37092 BAE38123
EMBL CAH92510 CAH93265 CAH93400
GB AAA58438 AAC50223 AAD34233 AAD38412 AAH06318
REF NP_001029603 NP_001107607 NP_001107608 NP_001107609 NP_001107610
SP Q08495 Q5R4B6 Q9WV69
TPG DAA26732