BMRB Entry 5969

Title:
Solution Structure of holo-Neocarzinostatin
Deposition date:
2003-10-13
Original release date:
2005-05-03
Authors:
Takashima, Hiroyuki; Ishino, Tetsuya; Yoshida, Takuya; Hasuda, Katsumi; Ohkubo, Tadayasu; Kobayashi, Yuji
Citation:

Citation: Takashima, Hiroyuki; Yoshida, Takuya; Ishino, Tetsuya; Hasuda, Katsumi; Ohkubo, Tadayasu; Kobayashi, Yuji. "Solution NMR structure investigation for releasing mechanism of neocarzinostatin chromophore from holoprotein."  J. Biol. Chem. 280, 11340-11346 (2005).
PubMed: 15640161

Assembly members:

Assembly members:
Neocarzinostatin, polymer, 113 residues, Formula weight is not available
CHR, non-polymer, 659.636 Da.

Natural source:

Natural source:   Common Name: Streptomyces carzinostaticus   Taxonomy ID: 1897   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptomyces carzinostaticus

Experimental source:

Experimental source:   Production method: purified from the natural source

Data sets:
Data typeCount
13C chemical shifts179
15N chemical shifts115
1H chemical shifts682

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Neocarzinostatin1
2Neocarzinostatin chromophore2

Entities:

Entity 1, Neocarzinostatin 113 residues - Formula weight is not available

1   ALAALAPROTHRALATHRVALTHRPROSER
2   SERGLYLEUSERASPGLYTHRVALVALLYS
3   VALALAGLYALAGLYLEUGLNALAGLYTHR
4   ALATYRASPVALGLYGLNCYSALATRPVAL
5   ASPTHRGLYVALLEUALACYSASNPROALA
6   ASPPHESERSERVALTHRALAASPALAASN
7   GLYSERALASERTHRSERLEUTHRVALARG
8   ARGSERPHEGLUGLYPHELEUPHEASPGLY
9   THRARGTRPGLYTHRVALASPCYSTHRTHR
10   ALAALACYSGLNVALGLYLEUSERASPALA
11   ALAGLYASNGLYPROGLUGLYVALALAILE
12   SERPHEASN

Entity 2, Neocarzinostatin chromophore - C35 H33 N O12 - 659.636 Da.

1   CHR

Samples:

sample_1: Neocarzinostatin, [U-90% 15N; U-90% 13C], 2.5 mM; NEOCARZINOSTATIN-CHROMOPHORE, [U-10% 13C], 2.5 mM

sample_cond_1: ionic strength: 0 M; pH: 5.0; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1not availablesample_cond_1
3D 13C-separated NOESYsample_1not availablesample_cond_1
2D NOESYsample_1not availablesample_cond_1
3D HNCAsample_1not availablesample_cond_1

Software:

X-PLOR vNIH 2.0.6 - refinement

NMR spectrometers:

  • Varian Unity 750 MHz
  • Bruker AMX 600 MHz

Related Database Links:

BMRB 1766 5343 5344 6888 695
PDB
DBJ BAA01764 BAB83894 BAB83895 BAB86365 BAB86366
GenBank AAB28103 AAM77994
SWISS-PROT P0A3R9 P0A3S0

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks