BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5971

Title: Letter to the Editor: Backbone and side chain resonance assignments of domain III of tick-borne Langat flavivirus Envelope protein   PubMed: 15243186

Authors: Mukherjee, Munia; Dutta, Kaushik; Pascal, Steven; Fox, Robert

Citation: Mukherjee, Munia; Dutta, Kaushik; Pascal, Steven; Fox, Robert. "Letter to the Editor: Backbone and side chain resonance assignments of domain III of tick-borne Langat flavivirus Envelope protein"  J. Biomol. NMR 29, 535-536 (2004).

Assembly members:
Langat E-D3, polymer, 108 residues, 11164 Da.

Natural source:   Common Name: Flaviviridae   Taxonomy ID: 11085   Superkingdom: viruses   Kingdom: Metazoa   Genus/species: Flavivirus Langat virus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Langat E-D3: GSEFGSKGLTYTVCDKTKFT WKRAPTDSGHDTVVMEVGFS GTRPCRIPVRAVAHGVPEVN VAMLITPNPTMENNGGGFIE MQLPPGDNIIYVGDLNHQWF QKGSSIGR

Data sets:
Data typeCount
13C chemical shifts417
1H chemical shifts591
15N chemical shifts114

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1LGT-E-D3 monomer1

Entities:

Entity 1, LGT-E-D3 monomer 108 residues - 11164 Da.

1   GLYSERGLUPHEGLYSERLYSGLYLEUTHR
2   TYRTHRVALCYSASPLYSTHRLYSPHETHR
3   TRPLYSARGALAPROTHRASPSERGLYHIS
4   ASPTHRVALVALMETGLUVALGLYPHESER
5   GLYTHRARGPROCYSARGILEPROVALARG
6   ALAVALALAHISGLYVALPROGLUVALASN
7   VALALAMETLEUILETHRPROASNPROTHR
8   METGLUASNASNGLYGLYGLYPHEILEGLU
9   METGLNLEUPROPROGLYASPASNILEILE
10   TYRVALGLYASPLEUASNHISGLNTRPPHE
11   GLNLYSGLYSERSERILEGLYARG

Samples:

sample_1: Langat E-D3, [U-100% 13C; U-100% 15N], 0.7 mM; Tris 10 mM; Bis-Tris 50 mM; NaCL 10 mM

sample_2: Langat E-D3, [U-99% 15N], 0.8 mM

cond_1: pH: 6.1; temperature: 298 K; ionic strength: 0.1 M

Experiments:

NameSampleSample stateSample conditions
15N-HSQCnot availablenot availablenot available
13C-HSQCnot availablenot availablenot available
HNCOnot availablenot availablenot available
CBCACONHnot availablenot availablenot available
HNCACBnot availablenot availablenot available
C(C0)NHnot availablenot availablenot available
HBHACONHnot availablenot availablenot available
HC(CO)NHnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
N15-NOESY-HSQCnot availablenot availablenot available
N15-TOCSY-HSQCnot availablenot availablenot available
C13-NOESY-HSQCnot availablenot availablenot available

Software:

NMRView v5.04 -

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 6800
PDB
GB AAA02740 AAF75259 AAF75260 ABH10022 ACH42698
REF NP_620108 NP_740295
SP P29837 P29838