BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5975

Title: 1H and 15N chemical shift assignments for the N-domain of trout cardiac troponin C at 30 and 7 C   PubMed: 12767223

Authors: Blumenschein, Tharin; Gillis, Todd; Tibbits, Glen; Sykes, Brian

Citation: Gillis, Todd; Blumenschein, Tharin; Sykes, Brian; Tibbits, Glen. "Effect of temperature and the F27W mutation on the Ca2+ activated structural transition of trout cardiac troponin C"  Biochemistry 42, 6418-6426 (2003).

Assembly members:
trout troponin C, polymer, 89 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:   Common Name: rainbow trout   Taxonomy ID: 8022   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Oncorhynchus mykiss

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
trout troponin C: MNDIYKAAVEQLTDEQKNEF KAAFDIFIQDAEDGCISTKE LGKVMRMLGQNPTPEELQEM IDEVDEDGSGTVDFDEFLVM MVRCMKDDS

Data sets:
Data typeCount
1H chemical shifts1100
13C chemical shifts292
15N chemical shifts186

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1trout N-terminal domin of cardiac TnC1
2CALCIUM (II) ION2

Entities:

Entity 1, trout N-terminal domin of cardiac TnC 89 residues - Formula weight is not available

1   METASNASPILETYRLYSALAALAVALGLU
2   GLNLEUTHRASPGLUGLNLYSASNGLUPHE
3   LYSALAALAPHEASPILEPHEILEGLNASP
4   ALAGLUASPGLYCYSILESERTHRLYSGLU
5   LEUGLYLYSVALMETARGMETLEUGLYGLN
6   ASNPROTHRPROGLUGLULEUGLNGLUMET
7   ILEASPGLUVALASPGLUASPGLYSERGLY
8   THRVALASPPHEASPGLUPHELEUVALMET
9   METVALARGCYSMETLYSASPASPSER

Entity 2, CALCIUM (II) ION - Ca - 40.078 Da.

1   CA

Samples:

sample_1: trout troponin C, [U-90% 15N], 1.34 mM; imidazole 10 mM; KCl 100 mM; dithiothreitol 20 mM; NaN3 0.03%; 2,2-dimethyl-2-silapentane-5-sulfonic_acid_(DSS) 0.2 mM

sample_2: trout troponin C, [U-96% 15N], 1.34 mM; imidazole 10 mM; KCl 100 mM; dithiothreitol 20 mM; NaN3 0.03%; 2,2-dimethyl-2-silapentane-5-sulfonic_acid_(DSS) 0.2 mM

conditions_1: pH: 6.9 na; temperature: 303 K

conditions_2: pH: 6.9 na; temperature: 280 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
15N DIPSI-HSQCnot availablenot availablenot available
15N NOESY-HSQCnot availablenot availablenot available
HNHAnot availablenot availablenot available
HNHBnot availablenot availablenot available
2D-NOESYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian Unity 600 MHz
  • Varian INOVA 800 MHz

Related Database Links:

PDB
EMBL CDQ82174
GB AAP33791 ACQ58284
REF NP_001117928 XP_003439031 XP_004546240 XP_005053154 XP_005448716