BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5987

Title: Interaction of the TAZ1 domain of CREB-binding protein with the activation domain of CITED2: Regulation by competition between intrinsically unstructured ligands for non-identical binding sites   PubMed: 14594809

Authors: De Guzman, Roberto; Martinez-Yamout, Maria; Dyson, H. Jane; Wright, Peter

Citation: De Guzman, Roberto; Martinez-Yamout, Maria; Dyson, H. Jane; Wright, Peter. "Interaction of the TAZ1 domain of the CREB-binding protein with the activation domain of CITED2: Regulation by competition between intrinsically unstructured ligands for non-identical binding sites"  J. Biol. Chem. 279, 3042-3049 (2004).

Assembly members:
CITED2 C-terminal activation domain, polymer, 51 residues, Formula weight is not available
CBP TAZ1 domain, polymer, 100 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
CITED2 C-terminal activation domain: MTDFIDEEVLMSLVIEMGLD RIKELPELWLGQNEFDFMTD FVCKQQPSRVS
CBP TAZ1 domain: ATGPTADPEKRKLIQQQLVL LLHAHKCQRREQANGEVRAC SLPHCRTMKNVLNHMTHCQA GKACQVAHCASSRQIISHWK NCTRHDCPVCLPLKNASDKR

Data sets:
Data typeCount
1H chemical shifts923
13C chemical shifts481
15N chemical shifts145

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CITED2 CAD1
2CBP TAZ12
3ZINC (II) ION, 13
4ZINC (II) ION, 23
5ZINC (II) ION, 33

Entities:

Entity 1, CITED2 CAD 51 residues - Formula weight is not available

1   METTHRASPPHEILEASPGLUGLUVALLEU
2   METSERLEUVALILEGLUMETGLYLEUASP
3   ARGILELYSGLULEUPROGLULEUTRPLEU
4   GLYGLNASNGLUPHEASPPHEMETTHRASP
5   PHEVALCYSLYSGLNGLNPROSERARGVAL
6   SER

Entity 2, CBP TAZ1 100 residues - Formula weight is not available

1   ALATHRGLYPROTHRALAASPPROGLULYS
2   ARGLYSLEUILEGLNGLNGLNLEUVALLEU
3   LEULEUHISALAHISLYSCYSGLNARGARG
4   GLUGLNALAASNGLYGLUVALARGALACYS
5   SERLEUPROHISCYSARGTHRMETLYSASN
6   VALLEUASNHISMETTHRHISCYSGLNALA
7   GLYLYSALACYSGLNVALALAHISCYSALA
8   SERSERARGGLNILEILESERHISTRPLYS
9   ASNCYSTHRARGHISASPCYSPROVALCYS
10   LEUPROLEULYSASNALASERASPLYSARG

Entity 3, ZINC (II) ION, 1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: CITED2 C-terminal activation domain, [U-13C; U-15N], 0.5 mM; CBP TAZ1 domain 0.5 mM; Tris-d11 10 mM; NaN3 2 mM; D2O 10%; H2O 90%

sample_2: CITED2 C-terminal activation domain 0.5 mM; CBP TAZ1 domain, [U-13C; U-15N], 0.5 mM; Tris-d11 10 mM; NaN3 2 mM; D2O 10%; H2O 90%

cond_1: pH: 6.8; temperature: 298 K; ionic strength: 16 mM

Experiments:

NameSampleSample stateSample conditions
3D HNCAnot availablenot availablecond_1
3D CBCACONHnot availablenot availablecond_1
3D HNCACBnot availablenot availablecond_1
3D HCCH-TOCSYnot availablenot availablecond_1
3D HCCH-COSYnot availablenot availablecond_1
3D 1H-15N NOESYnot availablenot availablecond_1
3D 1H-13C NOESYnot availablenot availablecond_1
2D HSQCnot availablenot availablecond_1
2D 1H-13C HMQCnot availablenot availablecond_1

Software:

NMRPipe -

NMR spectrometers:

  • BRUKER AVANCE 900 MHz
  • BRUKER AVANCE 800 MHz
  • BRUKER DRX 600 MHz

Related Database Links:

SWISS-PROT P45481 Q92793
REF NP_596872 XP_001095225 NP_001073315 NP_004371 NP_001020603
PRF 1923401A
GenBank AAC51770 AAH72594 AAC08447 AAC51331 AAB28651
DBJ BAG65526 BAE06125
PDB
BMRB 5327 6268