BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5991

Title: Backbone and Sidechain 1H, 13C, and 15N Chemical Shift Assignments and Backbone 15N Relaxation Parameters for Murine Ets-1 deltaN301   PubMed: 15591056

Authors: Lee, Gregory; Donaldson, Logan; Pufall, Miles; Kang, Hyun-Seo; Gentile, Lisa; Pot, Isabelle; Graves, Barbara; McIntosh, Lawrence

Citation: Lee, Gregory; Donaldson, Logan; Pufall, Miles; Kang, Hyun-Seo; Pot, Isabelle; Graves, Barbara; McIntosh, Lawrence. "The structural and dynamic basis of Ets-1 DNA Binding autoinhibition"  J. Biol. Chem. 280, 7088-7099 (2005).

Assembly members:
Ets-1, polymer, 140 residues, 16226.51 Da.

Natural source:   Common Name: House mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Ets-1: KGTFKDYVRDRADLNKDKPV IPAAALAGYTGSGPIQLWQF LLELLTDKSCQSFISWTGDG WEFKLSDPDEVARRWGKRKN KPKMNYEKLSRGLRYYYDKN IIHKTAGKRYVYRFVSDLQS LLGYTPEELHAMLDVKPDAD

Data sets:
Data typeCount
1H chemical shifts1025
13C chemical shifts657
15N chemical shifts156
T1 relaxation values125
T2 relaxation values125
order parameters124

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ets-1 DN301 monomer1

Entities:

Entity 1, Ets-1 DN301 monomer 140 residues - 16226.51 Da.

1   LYSGLYTHRPHELYSASPTYRVALARGASP
2   ARGALAASPLEUASNLYSASPLYSPROVAL
3   ILEPROALAALAALALEUALAGLYTYRTHR
4   GLYSERGLYPROILEGLNLEUTRPGLNPHE
5   LEULEUGLULEULEUTHRASPLYSSERCYS
6   GLNSERPHEILESERTRPTHRGLYASPGLY
7   TRPGLUPHELYSLEUSERASPPROASPGLU
8   VALALAARGARGTRPGLYLYSARGLYSASN
9   LYSPROLYSMETASNTYRGLULYSLEUSER
10   ARGGLYLEUARGTYRTYRTYRASPLYSASN
11   ILEILEHISLYSTHRALAGLYLYSARGTYR
12   VALTYRARGPHEVALSERASPLEUGLNSER
13   LEULEUGLYTYRTHRPROGLUGLULEUHIS
14   ALAMETLEUASPVALLYSPROASPALAASP

Samples:

sample_1: Ets-1, [U-99% 15N; U-99% 13C], 0.3 – 0.8 mM; phosphate buffer 20 mM; NaCl 500 mM; DTT 5 mM; NaN3 0.02%

sample_2: Ets-1, [U-99% 15N], 0.3 – 0.8 mM

sample_3: Ets-1, [U-98% 15N; U-98% 13C; U-97% 2H], 0.3 – 0.8 mM

sample_4: Ets-1, [U-99% 15N; U-10% 13C], 0.3 – 0.8 mM

Condition_1: pH: 6.5; temperature: 301 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
1H-13C CT HSQCnot availablenot availablenot available
1H-15N NOESY-HSQCnot availablenot availablenot available
1H-15N TOCSY-HSQCnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NNHnot availablenot availablenot available
HNHAnot availablenot availablenot available
HNHBnot availablenot availablenot available
HACANnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
HCC-TOCSYnot availablenot availablenot available
HNCOCAnot availablenot availablenot available
HNCOCACBnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCOnot availablenot availablenot available
EZ-HMQC-NH2not availablenot availablenot available
methyl CN NOESYnot availablenot availablenot available
simultaneous CN NOESYnot availablenot availablenot available

Software:

FELIX v2000 - spectral processing, peak peaking, peak assignments

NMRPipe - spectral processing

NMR spectrometers:

  • Varian Unity 500 MHz
  • Varian Inova 600 MHz
  • Varian Inova 800 MHz

Related Database Links:

SWISS-PROT P41156 P27577 P15062 P14921 P13474
REF NP_035938 NP_005229 NP_001092576 NP_001076130 NP_001033731
PRF 1613535A
GenBank AAA52410 AAA48764 AAA48669 AAA48668 AAA21093
EMBL CAE45783 CAA37904 CAA32904 CAA31442 CAA31441
DBJ BAE40197 BAC36266
PDB