BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5994

Title: Solution structure of Human Orexin-A:Regulator of Appetite and Wakefulness   PubMed: 15479620

Authors: Kim, H.-Y.; Hong, E.; Kim, J.-I.; Lee, W.

Citation: Kim, H.-Y.; Hong, E.; Kim, J.-I.; Lee, W.. "Solution structure of Human Orexin-A: Regulator of Appetite and Wakefulness"  J. Biochem. Mol. Biol. 37, 565-573 (2004).

Assembly members:
human orexin-A, polymer, 33 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
human orexin-A: QPLPDCCRQKTCSCRLYELL HGAGNHAAGILTL

Data sets:
Data typeCount
1H chemical shifts166

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Orexin-A1

Entities:

Entity 1, Orexin-A 33 residues - Formula weight is not available

1   GLNPROLEUPROASPCYSCYSARGGLNLYS
2   THRCYSSERCYSARGLEUTYRGLULEULEU
3   HISGLYALAGLYASNHISALAALAGLYILE
4   LEUTHRLEU

Samples:

sample_1: human orexin-A 2 mM; phosphate buffer 50 mM; H2O 90%; D2O 10%

sample_2: human orexin-A 2 mM; phosphate buffer 50 mM; D2O 100%

sample_cond_1: pH: 7.0; temperature: 278 K; ionic strength: 50 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available

Software:

CNS v1.0 - structure solution, refinement

XWINNMR v2.0 - processing

NMRPipe v1.7 - processing

Sparky v3.60 - data analysis

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 6441
PDB
DBJ BAB91446 BAG73274
GB AAC02933 AAC02934 AAC26827 AAC39600 AAC40039
REF NP_001029166 NP_001159992 NP_001181361 NP_001515 NP_034540
SP O43612 O55232 O55241 O77668 P56717
TPG DAA18421