BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6002

Title: Structure of the C-terminal domain of p63   PubMed: 16679535

Authors: Cadot, Bruno; Candi, Eleonora; Cicero, Daniel; Desideri, Alesssandro; Mele, Sonia; Melino, Gerry; Paci, Maurizio

Citation: Cicero, Daniel; Falconi, M.; Candi, Eleonora; Mele, Sonia; Cadot, Bruno; Di Venere, A.; Rufini, S.; Melino, Gerry; Desideri, Alessandro. "NMR structure of the p63 SAM domain and dynamical properties of G534V and T537P pathological mutants, identified in the AEC syndrome"  Cell Biochem. Biophys. 44, 475-489 (2006).

Assembly members:
polymer, polymer, 75 residues, 8379 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
polymer: PPPYPTDCSIVSFLARLGCS SCLDYFTTQGLTTIYQIEHY SMDDLASLKIPEQFRHAIWK GILDHRQLHEFAAAS

Data sets:
Data typeCount
13C chemical shifts337
15N chemical shifts81
1H chemical shifts507

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1p63 monomer1

Entities:

Entity 1, p63 monomer 75 residues - 8379 Da.

1   PROPROPROTYRPROTHRASPCYSSERILE
2   VALSERPHELEUALAARGLEUGLYCYSSER
3   SERCYSLEUASPTYRPHETHRTHRGLNGLY
4   LEUTHRTHRILETYRGLNILEGLUHISTYR
5   SERMETASPASPLEUALASERLEULYSILE
6   PROGLUGLNPHEARGHISALAILETRPLYS
7   GLYILELEUASPHISARGGLNLEUHISGLU
8   PHEALAALAALASER

Samples:

sample_1: polymer, [U-15N], 0.75 mM; sodium posphate 25 mM; sodium chloride 150 mM; AEBSF 1 mM; DTT 3 mM

sample_2: polymer, [U-13C; U-15N], 0.25 mM; sodium posphate 25 mM; sodium chloride 150 mM; AEBSF 1 mM; DTT 3 mM

Exp-cond_1: pH: 6.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCnot availablenot availablenot available
3D 1H-1H-15N TOCSYnot availablenot availablenot available
3D 1H-1H-15N NOESYnot availablenot availablenot available
3D HNHAnot availablenot availablenot available
3D HNCAnot availablenot availablenot available
3D HNCOnot availablenot availablenot available
3D CBCA(co)NHnot availablenot availablenot available
3D CBCANHnot availablenot availablenot available
3D HBHA(co)NHnot availablenot availablenot available
3D HACACOnot availablenot availablenot available
2D HACAnot availablenot availablenot available
3D hCCH TOCSYnot availablenot availablenot available
3D HcCH TOCSYnot availablenot availablenot available
3D HcCH COSYnot availablenot availablenot available
3D hCCH COSYnot availablenot availablenot available
3D C13,N15 edited NOESYnot availablenot availablenot available
2D 1H-13C HSQCnot availablenot availablenot available

Software:

NMRView v5 - peak-picking analysis of processed data

InsightII - graphic processing

X-PLOR v3.8 - Structure calculation

Procheck NMR - Structure validation

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 400 MHz

Related Database Links:

PDB
GB ELW50906
REF XP_006156783 XP_006156784