BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6004

Title: 1H, 13C, 15N assignments of human Cofilin   PubMed: 15213453

Authors: Zierler-Gould, K.; Pope, B.; Weeds, A.; Ball, L.

Citation: Zierler-Gould, K.; Pope, B.; Weeds, A.; Ball, L.. "Letter to the editor: Backbone and sidechain 1H, 13C and 15N resonance assignments of human cofilin "  J. Biomol. NMR 29, 429-430 (2004).

Assembly members:
cofilin, polymer, 166 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
cofilin: MASGVAVSDGVIKVFNDMKV RKSSTPEEVKKRKKAVLFCL SEDKKNIILEEGKEILVGDV GQTVDDPYATFVKMLPDKDC RYALYDATYETKESKKEDLV FIFWAPESAPLKSKMIYASS KDAIKKKLTGIKHELQANCY EEVKDRCTLAEKLGGSAVIS LEGKPL

Data sets:
Data typeCount
1H chemical shifts1166
13C chemical shifts534
15N chemical shifts164

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cofilin1

Entities:

Entity 1, cofilin 166 residues - Formula weight is not available

1   METALASERGLYVALALAVALSERASPGLY
2   VALILELYSVALPHEASNASPMETLYSVAL
3   ARGLYSSERSERTHRPROGLUGLUVALLYS
4   LYSARGLYSLYSALAVALLEUPHECYSLEU
5   SERGLUASPLYSLYSASNILEILELEUGLU
6   GLUGLYLYSGLUILELEUVALGLYASPVAL
7   GLYGLNTHRVALASPASPPROTYRALATHR
8   PHEVALLYSMETLEUPROASPLYSASPCYS
9   ARGTYRALALEUTYRASPALATHRTYRGLU
10   THRLYSGLUSERLYSLYSGLUASPLEUVAL
11   PHEILEPHETRPALAPROGLUSERALAPRO
12   LEULYSSERLYSMETILETYRALASERSER
13   LYSASPALAILELYSLYSLYSLEUTHRGLY
14   ILELYSHISGLULEUGLNALAASNCYSTYR
15   GLUGLUVALLYSASPARGCYSTHRLEUALA
16   GLULYSLEUGLYGLYSERALAVALILESER
17   LEUGLUGLYLYSPROLEU

Samples:

sample_1: cofilin, [U-15N; U-13C], 0.8 mM; phosphate buffer 10 mM; H2O 90%; D2O 10%

sample_2: cofilin, [U-15N], 1 mM; phosphate buffer 10 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 6.0; temperature: 300 K; ionic strength: 10 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESY-HSQC in H2O (aliphatic centred)not availablenot availablenot available
3D 13C-separated NOESY-HSQC in H2O (aromatic centred)not availablenot availablenot available
3D 15N-separated NOESY-HSQCnot availablenot availablenot available
3D HNHAnot availablenot availablenot available
3D HNHBnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
2D DQF-COSYnot availablenot availablenot available

Software:

XWINNMR v1.3 - acquisition, processing

AZARA v2.1 - processing, viewing

ANSIG v3.3 - assignment, integration, creation of NOE restraint lists

CNS v1.1 - structure solution, refinement

NMR spectrometers:

  • Bruker DMX 750 MHz

Related Database Links:

PDB
DBJ BAA00364 BAA00589 BAB29074 BAB32114 BAC34363
EMBL CAA44694 CAA64685
GB AAA31020 AAA64501 AAH11005 AAH12265 AAH12318
REF NP_001004043 NP_001009484 NP_001015655 NP_001253534 NP_005498
SP P10668 P18760 P23528 P45592 Q4R5C0
TPG DAA13572