BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6009

Title: NMR structure of the thrombin-binding DNA aptamer stabilized by Sr2+   PubMed: 15214802

Authors: Mao, X.; Marky, L.; Gmeiner, W.

Citation: Mao, X.; Marky, L.; Gmeiner, W.. "NMR structure of the thrombin-binding DNA aptamer stabilized by Sr2+"  J. Biomol. Struct. Dyn. 22, 25-33 (2004).

Assembly members:
thrombin binding 15mer DNA aptamer, polymer, 15 residues, 4600 Da.
SR, non-polymer, 87.620 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
thrombin binding 15mer DNA aptamer: GGTTGGTGTGGTTGG

Data sets:
Data typeCount
31P chemical shifts14

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Thrombin-binding DNA aptamer1
2Strontium (II) ion2

Entities:

Entity 1, Thrombin-binding DNA aptamer 15 residues - 4600 Da.

1   DGDGDTDTDGDGDTDGDTDG
2   DGDTDTDGDG

Entity 2, Strontium (II) ion - Sr - 87.620 Da.

1   SR

Samples:

sample_1: thrombin binding 15mer DNA aptamer 1 mM; SRONTIUM (II) ION1 – 1.3 mM; D2O 100%

sample_2: thrombin binding 15mer DNA aptamer 1 mM; SRONTIUM (II) ION1 – 1.3 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 3 mM; pH: 4.7; pressure: 1 atm; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablesample_cond_1
2D TOCSYnot availablenot availablesample_cond_1
E-COSYnot availablenot availablesample_cond_1
P31-H1 COSYnot availablenot availablesample_cond_1
ROESYnot availablenot availablesample_cond_1

Software:

CNS - refinement

NMR spectrometers:

  • Bruker AVANCE 600 MHz

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