BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6012

Title: Human Sgt1 binds to HSP90 through the CS domain and not the TPR domain   PubMed: 14761955

Authors: Lee, Young-Tae; Jacob, Jaison; Michowski, Wojciech; Nowotny, Marcin; Kuznicki, Jacek; Chazin, Walter

Citation: Lee, Young-Tae; Jacob, Jaison; Michowski, Wojciech; Nowotny, Marcin; Kuznicki, Jacek; Chazin, Walter. "Human Sgt1 binds to HSP90 through the CS domain and not the TPR domain"  J. Biol. Chem. 279, 16511-16517 (2004).

Assembly members:
Sgt1 CS, polymer, 114 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Sgt1 CS: GSHMTHQSKIKYDWYQTESQ VVITLMIKNVQKNDVNVEFS EKELSALVKLPSGEDYNLKL ELLHPIIPEQSTFKVLSTKI EIKLKKPEAVRWEKLEGQGD VPTPKQFVADVKNL

Data sets:
Data typeCount
13C chemical shifts497
1H chemical shifts749
15N chemical shifts115

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Sgt1 CS1

Entities:

Entity 1, Sgt1 CS 114 residues - Formula weight is not available

1   GLYSERHISMETTHRHISGLNSERLYSILE
2   LYSTYRASPTRPTYRGLNTHRGLUSERGLN
3   VALVALILETHRLEUMETILELYSASNVAL
4   GLNLYSASNASPVALASNVALGLUPHESER
5   GLULYSGLULEUSERALALEUVALLYSLEU
6   PROSERGLYGLUASPTYRASNLEULYSLEU
7   GLULEULEUHISPROILEILEPROGLUGLN
8   SERTHRPHELYSVALLEUSERTHRLYSILE
9   GLUILELYSLEULYSLYSPROGLUALAVAL
10   ARGTRPGLULYSLEUGLUGLYGLNGLYASP
11   VALPROTHRPROLYSGLNPHEVALALAASP
12   VALLYSASNLEU

Samples:

sample_1: Sgt1 CS, [U-15N], 1 – 2 mM; H2O 93%; D2O 7%

sample_2: Sgt1 CS, [U-15N; U-13C], 1 – 2 mM; H2O 93%; D2O 7%

sample_3: Sgt1 CS, [U-13C], 1 – 2 mM; D2O 100%

sample_4: Sgt1 CS1 – 2 mM; D2O 100%

Ex-cond_1: pH: 4.5; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1-2 MM SGT1 CS DOMAIN U-15N, 20 MM ACETIC ACID, 5 MM DTT, 93% H2O, 7% D2Onot availablenot availableEx-cond_1
2D 15N-1H HSQCnot availablenot availableEx-cond_1
3D 15N-edited NOESYnot availablenot availableEx-cond_1
1-2 MM SGT1 CS DOMAIN U-15N,U-13C, 20 MM ACETIC ACID, 5 MM DTT, 93% H2O, 7% D2Onot availablenot availableEx-cond_1

Software:

nmrpipe - processing

sparky - assignment

pipp - structure-assisted NOESY analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAF84091 BAF84817 BAG63688 BAI46473
EMBL CAC51433
GB AAD30062 AAH00911 AAP88800 AAQ01749 AAQ76039
REF NP_001124384 NP_006695 XP_001084394 XP_001084516 XP_002742679
SP Q9Y2Z0